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| 大黄鱼(Pseudosciaena crocea)精子冷冻前后的活力及超微结构变化 | |
| 引用本文： | 程 顺,闫家强,竺俊全,姜建湖,吴雄飞,史会来.大黄鱼(Pseudosciaena crocea)精子冷冻前后的活力及超微结构变化[J].海洋与湖沼,2013,44(1):56-61. |
| 作者姓名： | 程 顺 闫家强 竺俊全 姜建湖 吴雄飞 史会来 |
| 作者单位： | 1. 宁波大学教育部应用海洋生物技术重点实验室 宁波315211 2. 宁波市海洋与渔业研究院 宁波 315012 3. 浙江省海洋水产研究所 舟山 316100 |
| 基金项目： | 国家星火计划项目, 2011GA701001 号; 浙江省水产新品种选育专项, 2012C12907-8 号; 宁波市科技计划重大项目, 2011C11005号; 宁波市科技创新团队项目, 2011B82018 号; 宁波大学优秀学位论文培育基金，PY2012016号; 舟山市科技计划项目, 10206 号 |
| 摘 要： | 采用两步降温法超低温冷冻保存大黄鱼精子,并用透射电镜技术研究了精子的超微结构损伤.结果表明,大黄鱼冻精的激活率、运动时间及寿命与鲜精相比无显著差异.鲜精中28.5％的精子形态结构异常,冻精中37％的精子形态结构异常.形态结构正常的精子表现为质膜与核膜结构完整、无膨胀现象,袖套、轴丝及中心粒结构正常,线粒体形态完整、嵴较发达；形态结构异常的精子表现为质膜破裂、脱落,质膜膨胀,核膜破裂、脱落,核局部受损伤,线粒体膨胀、嵴退化或消失,线粒体移位或脱落.结果显示,以Cortland溶液为稀释液,10％ DMSO为抗冻剂,对大黄鱼精子具有较好的抗冻保护作用. |
| 关 键 词： | 大黄鱼 精子 超低温冻存 活力 超微结构 |
| 收稿时间： | 2012/1/12 0:00:00 |
| 修稿时间： | 4/6/2012 12:00:00 AM |
VITALITY AND ULTRASTRUCTURE OBSERVATION OF FRESH AND CRYOPRESERVATED SPERM IN PSEUDOSCIAENA CROCEA |
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| CHENG Shun,YAN Jia-Qiang,ZHU Jun-Quan,JIANG Jian-Hu,WU Xiong-Fei and SHI Hui-Lai.VITALITY AND ULTRASTRUCTURE OBSERVATION OF FRESH AND CRYOPRESERVATED SPERM IN PSEUDOSCIAENA CROCEA[J].Oceanologia Et Limnologia Sinica,2013,44(1):56-61. | |
| Authors: | CHENG Shun YAN Jia-Qiang ZHU Jun-Quan JIANG Jian-Hu WU Xiong-Fei and SHI Hui-Lai |
| Institution: | Key Laboratory of Applied Marine Biotechnology by the Ministry of Education, Ningbo University;Key Laboratory of Applied Marine Biotechnology by the Ministry of Education, Ningbo University;Key Laboratory of Applied Marine Biotechnology by the Ministry of Education, Ningbo University;Key Laboratory of Applied Marine Biotechnology by the Ministry of Education, Ningbo University;Ningbo Academy of Oceanology and Fisheries;Marine Fishery Institute of Zhejiang Province |
| Abstract: | A two-step cooling procedure was employed to cryopreserve Pseudosciaena crocea sperm, and the sperm ultrastructure after which was observed under transmission electron microscopy. The results show that there were no significant differences between frozen-thawed sperm and fresh sperm by comparing the activation rate, moving time and life-span. Both the fresh sperm and cryopreserved sperm had ultrastructural damages in various degree. The deformation rate of the fresh and cryopreservated sperms were 28.5% and 37%, respectively. The following aspects of cryopreserved sperm with normal morphology was observed: the plasma and nuclear membrane; the sleeve, axoneme and centriole; the mitochondrion obtained integrity and with well-developed cristae. On the contrary, the sperm cryodamages were observed as follows: swelled or disrupted plasma and nuclear membrane; partially damaged nucleus; the swelled, dislocated or disarticulated mitochondrion with degenerated or vanished cristae. The results show that Cortland solutions and 10% DMSO are the best choice for extender and cryoprotecant, which are helpful for improving the frozen-thawed P. crocea sperm quality. |
| Keywords: | Pseudosciaena crocea Sperm Cryopreservation Vitality Ultrastructure |
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