| 液质联用技术在测定浮游植物色素与粒级分类中的应用 |
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| 引用本文： | 张晶晶,刘东艳,孙慧慧,王晓峰.液质联用技术在测定浮游植物色素与粒级分类中的应用[J].海洋通报,2018(2):158-164. |
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| 作者姓名： | 张晶晶 刘东艳 孙慧慧 王晓峰 |
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| 作者单位： | 中国科学院烟台海岸带研究所中国科学院海岸带环境过程与生态修复重点实验室;中国科学院大学;鲁东大学资源与环境工程学院;华东师范大学河口海岸学国家重点实验室 |
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| 基金项目： | 科技基础性工作专项（2014FY210600）；国家自然科学基金（41376121）；山东省自然科学基金委杰出青年基金项目（JQ201414） |
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| 摘 要： | 本研究为探讨液质联用技术在测定黄海浮游植物特征色素含量与粒级分类中应用的可行性,在黄海47个站位取表、中、底三层水样进行了分析,对比了超高效液相色谱(UHPLC)和超高效液相色谱串联三重四级杆质谱(UHPLC-MS/MS)两种仪器的色素检测结果。研究发现UHPLC-MS/MS的灵敏度更高,但多数情况下两种方法检测结果吻合度较好,均适用于黄海色素样品的测定。叶绿素a(Chl a)浓度和浮游植物粒级结构空间分析结果显示,山东半岛近岸和长江口东北侧海域,Chl a浓度较高,小型浮游植物占优势;黄海冷水团海域,Chl a浓度较低,微型和微微型浮游植物比例明显升高。该航次的Chl a绝对浓度和不同粒径浮游植物的贡献率与历史数据相比存在一定差异,这与调查时间、站位以及粒级分析方法的差异有关,需进一步比较研究。
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| 关 键 词： | 黄海 Chl a 浮游植物特征色素 粒级结构 UHPLC-MS/MS |
| 收稿时间： | 2017/3/10 0:00:00 |
| 修稿时间： | 2017/9/28 0:00:00 |
Application of UHPLC-MS/MS in determination of the phytoplankton pigment and size fraction |
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| ZHANG Jing-jing,LIU Dong-yan,SUN Hui-hui and WANG Xiao-feng.Application of UHPLC-MS/MS in determination of the phytoplankton pigment and size fraction[J].Marine Science Bulletin,2018(2):158-164. |
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| Authors: | ZHANG Jing-jing LIU Dong-yan SUN Hui-hui and WANG Xiao-feng |
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| Institution: | Key Laboratory of Coastal Environmental Processes and Ecological Remediation of CAS, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; University of Chinese Academy of Sciences, Beijing 100049, China; Department of Resources and Environmental Engineering, Ludong University,Yantai 264025, China,Key Laboratory of Coastal Environmental Processes and Ecological Remediation of CAS, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai 200062, China,State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai 200062, China and Key Laboratory of Coastal Environmental Processes and Ecological Remediation of CAS, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; University of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | In order to explore the feasibility of applying ultra-high performance liquid chromatography tandem triple quadrupole mass spectrometry (UHPLC-MS/MS) on pigment analysis and size fraction monitoring, seawater samples collected from 3 different water layers at 47 sites in the Yellow Sea were analyzed, it in terms of pigment concentrations acquired by ultra-high performance liquid chromatography (UHPLC) and UHPLC-MS/MS respectively and results got from the two ways were compared. No observious differences were observed from the result comparisons between the two methods for most cases although UHPLC-MS/MS were more sensitive, and the two methods were both suitable for pigment detection in the Yellow Sea. Based on the pigment results provided by UHPLC-MS/MS, the spatial distribution of chlorophyll a (Chl a) and size fraction were studied. The concentration of Chl a was much higher in the northeast of Yangtze River mouth and coastal waters of Shandong Peninsula, where micro phytoplankton was the dominant size fraction. The concentration of Chl a was much lower in the central Yellow Sea, and the contribution of nano- and pico- phytoplankton increased significantly in this region. Compared with previous studies, the absolute concentrations of Chl a and contribution of size fractions in our study showed some differences, which may be caused by the differences of investigation time, sites or analytical methods. Thus, more comparison study is still needed to further probe the reason behind these differences. |
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| Keywords: | Yellow Sea Chl a phytoplankton marker pigments size fraction UHPLC-MS/MS |
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