| 一株产铁载体细菌的筛选及其与云母的相互作用 |
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| 引用本文： | 何琳燕,张垠,盛下放,黄智.一株产铁载体细菌的筛选及其与云母的相互作用[J].高校地质学报,2012,18(1):117-124. |
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| 作者姓名： | 何琳燕 张垠 盛下放 黄智 |
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| 作者单位： | 南京农业大学 生命科学学院，农业部 农业环境微生物工程重点开放实验室，南京 210095 |
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| 摘 要： | 产铁载体细菌与云母类矿物相互作用的研究有助于了解矿物生物风化和土壤形成的演化规律和机理。采用纯培养法
自南京龙山废钾矿区酸模根际土壤分离筛选到一株高产荧光铁载体的细菌Z6，通过16S rDNA 序列分析和生理生化反应将其
鉴定为假单胞菌属（Pseudomonas sp.）；通过室温静置培养试验研究Z6 菌株与云母的相互作用结果表明，细菌生长代谢前期
（8~15 d）快速产生大量铁载体，菌株与矿物共培养75 d 后，产铁载体细菌对云母类矿物确实产生了风化作用，黑云母组发
酵液中Fe 含量增加了211 倍，Si 含量增加了约27.8 倍，SEM 显示云母表面出现溶蚀坑，细菌可以在矿物表面形成生物膜或
矿物- 细菌复合物。不同矿物对共培养体系中细菌数量、荧光铁载体含量、溶液pH 的影响不同。
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| 关 键 词： | 荧光铁载体 细菌 云母类矿物 生物风化作用 |
| 收稿时间： | 2011-08-07 |
| 修稿时间： | 2011-09-20 |
Isolation of a Siderophore-Producting Bacterial Strain and Mica-bacterial Interactions |
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| HE Linyan,ZHANG Yin,SHENG Xiafang,HUANG Zhi.Isolation of a Siderophore-Producting Bacterial Strain and Mica-bacterial Interactions[J].Geological Journal of China Universities,2012,18(1):117-124. |
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| Authors: | HE Linyan ZHANG Yin SHENG Xiafang HUANG Zhi |
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| Institution: | College of Life Sciences, Nanjing Agricultural University, Key Laboratory of Microbiological Engineering of;
Agricultural Environment, Ministry of Agriculture, Nanjing 210095, China |
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| Abstract: | studies on the interactions between siderophore-producting bacteria and mica minerals will help us understand the
mechanism of bio-weathering, the formation of soil, global cycle of several elements, and local environmental contamination. A siderophore(pyoverdins)-producting bacterial strain Z6 was isolated from rhizosphere soil of advantage wild plants sheep sorrel (Rumex acetosa L.) growing in Longshan potassium mine tailings in Nanjing, which was identified as Pseudomonas sp. by checking the individual morphology, colony characteristics, and 16S rDNA sequencing. Using the test cultures containing biotite or muscovite inoculated with Pseudomonas sp. Z6, we found that a strong increase in the amount of siderophore in the fiest 15 days and bacteria could influence silicon and iron mobilization from mica minerals consistently until 75 d of culture. The amounts of iron released from biotite in the presence of Z6 increased by 211 times and the silicon increased by a factor of 27.8, much higher than that in the negative control without minerals. SEM analysis revealed the formation of erosion and bacteria-mineral aggregates on the surface of mica. Cellular growth, siderophere production and pH change by Pseudomonas sp. Z6 cultivated in biotite were directly and quickly influenced, more significantly than that in muscovite experimental setup. The siderophore(pyoverdins)- producting bacterial strain Z6 might play an important role in the process of mica weathering. Production of bacterial siderophore may be related to the presence of different mica minerals. |
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| Keywords: | pyoverdins bacteria mica minerals bio-weathering |
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