| 红藻真江篱质粒的发现 |
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| 引用本文： | 秦松,童顺,崔武,王希华,李亚非,吴光耀,张培军,曾呈奎.红藻真江篱质粒的发现[J].海洋与湖沼,1994,25(4):349-352. |
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| 作者姓名： | 秦松 童顺 崔武 王希华 李亚非 吴光耀 张培军 曾呈奎 |
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| 作者单位： | 中国科学院海洋研究所!青岛，266071，中国科学院海洋研究所!青岛，266071，北京大学!北京，100871，中国科学院海洋研究所!青岛，266071，中国人民解放军肝病研究所!北京，100700，北京大学!北京，100871，中国科学院海洋研究所!青岛，266071，中国科学院海洋研究所!青岛，266071 |
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| 基金项目： | 中科院海洋研究所所长基金 |
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| 摘 要： | 于1992年4月一1993年4月，用液氮研磨-苯酚／氯仿抽提-异丙醇沉淀的方法提取青岛沿海产紫菜、真江蓠、龙须菜、海膜4种红藻的总DNA；利用Hoechst－CsCl密度梯度起离心法将叶绿体DNA与核DNA分开。对抽取的叶绿体DNA梯度组分电泳结果表明，真江蓠样品出现质粒的电泳条带。以透射电镜观察到闭会环状DNA图像（周长平均值为4.3×10－7m，约为1．3kb）；分子杂交结果显示，真江蓠质位与其叶绿体DNA之间没有同源性，而与核DNA之间有一定同源性。
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| 关 键 词： | 红藻 真江蓠 质粒 CsCl密度离心 |
| 收稿时间： | 1994/1/27 0:00:00 |
| 修稿时间： | 1994/3/18 0:00:00 |
PLASMID FROM THE RED ALGA GRACILARIA ASIATICA OF QINGDAO COAST IN CHINA |
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| Qin Song,Tong Shun,Cui Wu,Wang Xihu,Li Yafei,Wu Guangyao,Zhang Peijun and Zeng Chengkui ;.PLASMID FROM THE RED ALGA GRACILARIA ASIATICA OF QINGDAO COAST IN CHINA[J].Oceanologia Et Limnologia Sinica,1994,25(4):349-352. |
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| Authors: | Qin Song Tong Shun Cui Wu Wang Xihu Li Yafei Wu Guangyao Zhang Peijun and Zeng Chengkui ; |
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| Institution: | Institute of Oceanology, Academia Sinica, Qingdao 266071;Institute of Oceanology, Academia Sinica, Qingdao 266071;Department of Biology, Beijing, University, Beijing 100871;Institute of Oceanology, Academia Sinica, Qingdao 266071;Institute for Liver Diseases, PLA, Beijing 100700;Department of Biology, Beijing, University, Beijing 100871;Institute of Oceanology, Academia Sinica, Qingdao 266071;Institute of Oceanology, Academia Sinica, Qingdao 266071 |
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| Abstract: | DNAs of the rhodophyte, Gracilaria asiatica, Gracilariopsis lemaneiformis,Porphyra sp. and Halymenia sp. from coast of Qingdao were characterized using Hoechst 33258-Cesium chloride ultracentrifugation from 1992 to 1993. only in G.asiatica, an A-T rich plasmid was co-isolated while it co-migrated with chloroplast DNA in Hoechst-CsCl gradient. Circular and closed pattern was observed under an electron microscope. The girth was 4.3 X10-7m on average, and its size was ca.1300bp. Both gel electrophresis and hybridization showed that its copy number was high. It has homologous sequence with nuclear DNA, instead of chloroplast DNA,which suggest that it may have integrated form. |
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| Keywords: | Rhodophyte Gracilaria asiatica Plasmid Cesium chloride gradien tultracentrifugation |
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