| 静水压力诱导牙鲆( Paralichthys olivaceus)四倍体的条件优化 |
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| 引用本文： | 衣启麟,于海洋,王兴莲,王志刚,王旭波,齐 洁,张全启.静水压力诱导牙鲆( Paralichthys olivaceus)四倍体的条件优化[J].海洋与湖沼,2012,43(2):382-388. |
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| 作者姓名： | 衣启麟 于海洋 王兴莲 王志刚 王旭波 齐 洁 张全启 |
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| 作者单位： | 中国海洋大学海洋生命学院海洋生物遗传育种教育部重点实验室,青岛,266003 |
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| 基金项目： | 山东省良种工程重大课题“优质抗病速生鱼类良种选育”, 2008 —2014; 国家863 计划课题“海洋动物细胞分子育种关键技术”, SS2012AA100802 号; 山东省海洋生物学泰山学者岗位经费资助, ts2010021 号 |
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| 摘 要： | 采用静水压力抑制受精卵第一次卵裂的方法,进行了诱导牙鲆四倍体的研究。结果表明,将受精卵保持在(15.5±0.5)℃,从受精后70min开始用55MPa的压力处理6min后,正常仔鱼孵化率最高达到15.6%,此时的四倍体诱导率也最高达到63.3%。利用获得的最佳诱导条件处理获得了数千尾体长8—15cm的幼鱼,流式细胞仪检测和红血球长径测量结果显示,处理组幼鱼中四倍体约占13.3%,说明四倍体培育成功。该成果为开展牙鲆多倍体育种奠定了基础。
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| 关 键 词： | 牙鲆 四倍体 静水压力 多倍体育种 |
| 收稿时间： | 2010/11/25 0:00:00 |
| 修稿时间： | 2011/1/27 0:00:00 |
PRODUCTION OF VIABLE TETRAPLOID OLIVE FLOUNDER (PARALICHTHYS OLIVACEUS) BY HYDROSTATIC PRESSURE SHOCK |
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| YI Qi-Lin,YU Hai-Yang,WANG Xing-Lian,WANG Zhi-Gang,WANG Xu-Bo,QI Jie and ZHANG Quan-Qi.PRODUCTION OF VIABLE TETRAPLOID OLIVE FLOUNDER (PARALICHTHYS OLIVACEUS) BY HYDROSTATIC PRESSURE SHOCK[J].Oceanologia Et Limnologia Sinica,2012,43(2):382-388. |
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| Authors: | YI Qi-Lin YU Hai-Yang WANG Xing-Lian WANG Zhi-Gang WANG Xu-Bo QI Jie and ZHANG Quan-Qi |
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| Institution: | College of Marine Life Science, Ocean University of China; Key Laboratory of Marine Genetics and Breeding, Ministry of Education;College of Marine Life Science, Ocean University of China; Key Laboratory of Marine Genetics and Breeding, Ministry of Education;College of Marine Life Science, Ocean University of China; Key Laboratory of Marine Genetics and Breeding, Ministry of Education;College of Marine Life Science, Ocean University of China; Key Laboratory of Marine Genetics and Breeding, Ministry of Education;College of Marine Life Science, Ocean University of China; Key Laboratory of Marine Genetics and Breeding, Ministry of Education;College of Marine Life Science, Ocean University of China; Key Laboratory of Marine Genetics and Breeding, Ministry of Education;College of Marine Life Science, Ocean University of China; Key Laboratory of Marine Genetics and Breeding, Ministry of Education |
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| Abstract: | Viable tetraploid olive flounder(Paralichthys olivaceus) were hatched under hydrostatic pressure shock.The result showed that optimal hatching rate for tetraploid individuals was achieved by 6 min of pressure shock(55Mpa) at(15.5±0.5)℃,70min after fertilization.Under these conditions,the hatching rate of the treated eggs was 15.6%.Chromosome observation and counting of maximum number of nucleolus showed that the tetraploid rate was 63.3%.Several thousands of young fish with body length of 8—15cm were obtained using this method.Both flow-cytometry and erythrocyte diameter proved that 13.3% of the young fish were tetraploids.The successful production of tetraploid individuals provided possibilities for future polyploid breeding using this fish species. |
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| Keywords: | Paralichthys olivaceus Tetraploid Hydrostatic pressure shock Polyploid breeding |
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