Cloning and sequencing of cytochrome P450 2X1 from channel catfish (Ictalurus punctatus) |
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| Authors: | Schlenk D Furnes B Zhou X Debusk B C |
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| Institution: | Department of Environmental Sciences, University of California, Riverside 92521, USA. daniel.schlenk@ucr.edu |
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| Abstract: | Previous purification and immunochemical studies in livers of channel catfish indicated the presence of at least four cytochrome P450 (CYP) 2-like isoforms. Sequencing of the first 18 amino acids of one purified form indicated a CYP2 isoform. From this N-terminal sequence and other published CYP2 sequences from fish, primers were designed and a full-length CYP cDNA was identified from reverse-transcribed catfish liver mRNA. 5' and 3' RACE was used to obtain an open reading frame of 1470 bp encoding a 490 amino acid protein (approximately 57 kD). CYP2X1 was most identical to Fundulus heteroclitus CYP2P2 (41%); CYP2N2 (40%): and CYP2N1 (39%). |
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