| 基于线粒体COI基因序列探讨鸭嘴舌形贝地理分布 |
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| 引用本文： | 吴恋娟,盛桂莲,赖旭龙,侯新东,袁俊霞.基于线粒体COI基因序列探讨鸭嘴舌形贝地理分布[J].地质科技情报,2010,29(1). |
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| 作者姓名： | 吴恋娟 盛桂莲 赖旭龙 侯新东 袁俊霞 |
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| 作者单位： | 中国地质大学生物地质与环境地质教育部重点实验室,武汉,430074 |
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| 基金项目： | 国家自然科学基金项目(40672006;40902008);;生物地质与环境地质教育部重点实验室开放基金项目(BGEG0604) |
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| 摘 要： | 从河北秦皇岛采集的用无水乙醇保存的鸭嘴舌形贝(Lingula anatina)样品中提取了DNA,经PCR扩增后进行克隆,得到了2条长度为682 bp的线粒体细胞色素C氧化酶第I亚基(COI)基因片段。调用GenBank中日本有明海和中国香港的鸭嘴舌形贝同源序列,用邻接法和贝叶斯法构建了系统发育树,结果表明:秦皇岛地区的鸭嘴舌形贝与日本有明海的鸭嘴舌形贝形成姊妹群,具有较近的亲缘关系,与香港的鸭嘴舌形贝亲缘关系较远。初步分析认为西太平洋地区鸭嘴舌形贝现有的地理分布并不是由浮游幼虫的扩散引起的,不同地区鸭嘴舌形贝的差异可能跟地理隔离有关。
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| 关 键 词： | 鸭嘴舌形贝 线粒体COI基因 地理隔离 |
Geographical Pattern Analysis of the Brachiopod Lingula anatina Based on Mitochondrial COI Gene Sequences |
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| WU Lian-juan,SHENG Gui-lian,LAI Xu-long,HOU Xin-dong,YUAN Jun-xia.Geographical Pattern Analysis of the Brachiopod Lingula anatina Based on Mitochondrial COI Gene Sequences[J].Geological Science and Technology Information,2010,29(1). |
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| Authors: | WU Lian-juan SHENG Gui-lian LAI Xu-long HOU Xin-dong YUAN Jun-xia |
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| Institution: | Key Laboratory of Biogeology and Environmental Geology of Ministry of Education;China University of Geosciences;Wuhan 430074;China |
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| Abstract: | The mitochondrial cytochrome oxidase subunit I(COI) gene fragments(682 bp) were amplified and cloned from two pure alcohol preserved Lingula anatina specimens collected from Qinhuangdao,northern China.Homologous sequences of individuals from two other northwestern Pacific locations,Hong Kong(southern China) and Ariake Sea(southern Japan),were drawn from GenBank for phylogenetic analysis.Sequences of individuals from Qinhuangdao and Ariake Sea were grouped together in the phylogenetic tree,suggesting a close... |
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| Keywords: | Lingula anatina mitochondrial COI gene geographic isolation |
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