| Antioxidation and ATPase activity in the gill of mud crab Scylla serrata under cold stress |
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| 作者姓名： | 孔祥会 王桂忠 李少菁 |
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| 作者单位： | Department of Oceanography State Key Laboratory for Marine Environmental Science，Xiamen University，Department of Oceanography State Key Laboratory for Marine Environmental Science，Xiamen University，Department of Oceanography State Key Laboratory for Marine Environmental Science，Xiamen University，Xiamen 361005 China，College of Life Science Henan Normal University Xinxiang 453007 China，Xiamen 361005 China，Xiamen 361005 China |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | Mud crab (Scylla serrata) is an important commercial crustacean in China. An experiment was designed to study the effect of cold stress on S. serrata. After a one-week adaptation at 28oC, the temperature is suddenly reduced to 4oC. The crabs were sampled every 2 h for 10 h and dissected immediately to measure the enzyme activity. The crabs at room temperature (28oC) were used as the control group. The activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX), the content of malondialdehyde (MDA) and the activity of 4 ATPases (Na , K -ATPase; Mg2 -ATPase; Ca2 -ATPase; Ca2 , Mg2 -ATPase) were measured biochemically. In contrast to the control group, the SOD activity increased significantly from 2 to 6 h after the cold stress, and then decreased. The CAT and GPX activities increased in 2 h, and then decreased gradually. The content of MDA increased gradually in 4 h. The activity of Na , K -ATPase decreased in 2 h, increased up to the top value at Hour 6, then decreased again. The activities of Mg2 -ATPase, Ca2 -ATPase and Ca2 , Mg2 -ATPase increased significantly in 6 h, insignificantly in any other hours. Under cold stress, the activity of antioxidative enzymes in S. serrata was reduced at first then stabilized, ROS-scavenging weakened, and MDA accumulated gradually in the gill after 6 h. The activity of the 4 ATPases in the crab decreased after 6 h, suggesting that the ability to regulate ion concentration has been paralyzed. Therefore, the maximum period to sustain healthy meat in the crab under cold stress is 6 hours.
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| 关 键 词： | 锯缘青蟹 鳃部 冷应激 抗氧化作用 ATP酶活性 |
| 收稿时间： | 2005-07-11 |
| 修稿时间： | 2005-09-21 |
Antioxidation and ATPase activity in the gill of mud crab <Emphasis Type="Italic">Scylla serrata</Emphasis> under cold stress |
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| Kong?Xianghui,Wang?Guizhong,Li?Shaojing.Antioxidation and ATPase activity in the gill of mud crab Scylla serrata under cold stress[J].Chinese Journal of Oceanology and Limnology,2007,25(2):221-226. |
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| Authors: | Kong Xianghui Wang Guizhong Li Shaojing |
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| Institution: | (1) Department of Oceanography, State Key Laboratory for Marine Environmental Science, Xiamen University, Xiamen, 361005, China;(2) College of Life Science, Henan Normal University, Xinxiang, 453007, China |
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| Abstract: | Mud crab (Scylla serrata) is an important commercial crustacean in China. An experiment was designed to study the effect of cold stress on S. serrata. After a one-week adaptation at 28oC, the temperature is suddenly reduced to 4oC. The crabs were sampled every 2 h for 10 h and dissected immediately to measure the enzyme activity. The crabs at room temperature (28oC) were used as the control group. The activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX), the content of malondialdehyde (MDA) and the activity of 4 ATPases (Na , K -ATPase; Mg2 -ATPase; Ca2 -ATPase; Ca2 , Mg2 -ATPase) were measured biochemically. In contrast to the control group, the SOD activity increased significantly from 2 to 6 h after the cold stress, and then decreased. The CAT and GPX activities increased in 2 h, and then decreased gradually. The content of MDA increased gradually in 4 h. The activity of Na , K -ATPase decreased in 2 h, increased up to the top value at Hour 6, then decreased again. The activities of Mg2 -ATPase, Ca2 -ATPase and Ca2 , Mg2 -ATPase increased significantly in 6 h, insignificantly in any other hours. Under cold stress, the activity of antioxidative enzymes in S. serrata was reduced at first then stabilized, ROS-scavenging weakened, and MDA accumulated gradually in the gill after 6 h. The activity of the 4 ATPases in the crab decreased after 6 h, suggesting that the ability to regulate ion concentration has been paralyzed. Therefore, the maximum period to sustain healthy meat in the crab under cold stress is 6 hours. |
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| Keywords: | antioxidation ATPase gill cold stress Scylla serrata |
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