Tetraploid induction by inhibiting mitosis I in scallopChlamys farreri |
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| Authors: | Yang Hui-ping Guo Xi-ming Chen Zai-zhong Wang Yong-ping |
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| Institution: | (1) Experiment Marine Biology Laboratory, Institute of Oceanology, Chinese Academy of Sciences, 266071 Qingdao, China;(2) Haskin Shellfish Research Laboratory, Rutgers University, 6959 Miller Avenue, 08349 Port Norris, NJ, USA;(3) Fisheries College, Ocean University of Qingdao, 266003 Qingdao, China |
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| Abstract: | Tetraploid induction was carried out by inhibiting mitosis I in fertilized eggs ofChlamys farreri. Mitosis I was blocked with cold shock (5–7°C), Cytochalasin B (0.75 mg/L) and 6-dimethylaminopurine (6-DMAP) (60–75 mg/L)
when 60% fertilized eggs released polar body II at 20°C. At 4-cells embryo stage, the ploidy was determined by counting chromosome
number. In control groups, most embryos were diploids (72.22%) and aneuploids (24.78%). In Cytochalasin B, cold shock and
6-DMAP treated groups, tetraploids were respectively 10.51%, 4.08%, and 13.34%; aneuploids were 43.10%, 35.93% and 29.16%,
and triploids were 7.84%, 8.52% and 18.33%. At D-larva stage, ploidy was determined by flow cytometry (FCM). The ploidy analysis
of day 2 larvae showed diploids in control group and also in three treated groups. Juvenile scallops (0.2–0.3cm) which were
harvested in two control groups and two CB treated groups were all diploids through checking ploidy individually by FCM.
Contribution No. 3648 from the Institute of Oceanology, Chinese Academy of Sciences.
Contribution No. 238 from the Experimental Marine Biology Laboratory, Chinese Academy of Sciences.
Project funded by grant (No. 819-01-07) from the Chinese Science and Technology Ministry and also by “Hundred Talents Plan”
grant from the Chinese Academy of Sciences. |
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| Keywords: | scallopChalamys farreri tetraploid Cytochalasin B mitosis I |
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