| Isolation of Protoplasts from Undaria pinnatifida by Alginate Lyase Digestion |
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| 作者姓名： | HU Xiaoke JIANG Xiaolu* GUAN Huashi Fishery College Ocean University of China Qingdao P..R.China |
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| 作者单位： | HU Xiaoke，JIANG Xiaolu*，GUAN Huashi Fishery College，Ocean University of China，Qingdao 266003，P..R.China |
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| 摘 要： | 1 Introduction Protoplastsserveasabasicandversatiletoolforge neticengineeringandbiochemicalresearchforseveralreasons :theymayregeneratetheirwallsandprovideamodelsystemforstudyingwallbiogenesis ,theirlysisprovidesagentlemeansofpreparingsubcellularor ganelles ,andthemembraneexpositionallowsgeneticmanipulationsinvolvingfusionoruptakeofnucleicacidstobepossible .Theseadvantagesarelessachiev ablewhenusingintactcells (Liciaetal.,1999) .Incomparisonwithlandplantsandunicellularalgae ,on lylimitedrepo…
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Isolation of Protoplasts from Undaria pinnatifida by Alginate Lyase Digestion |
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| HU Xiaoke,JIANG Xiaolu*,GUAN Huashi Fishery College,Ocean University of China,Qingdao ,P..R.China.Isolation of Protoplasts from Undaria pinnatifida by Alginate Lyase Digestion[J].Journal of Ocean University of China,2003,2(1). |
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| Authors: | Hu Xiaoke Jiang Xiaolu GUAN Huashi |
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| Abstract: | The aim of this study is to isolate protoplasts from Undaria pinnatifida. Protoplasts of the alga were isolated enzymatically by using alginate lyase, which was prepared by fermenting culture of a strain Vibrio sp. 510. Monofacterial method was applied for optimizing digestion condition. The optimum condition for protoplast preparation is enzymatic digestion at 28 ℃ for 2 h using alginate lyase at the concentration of 213.36 U (8 mL) every 0.5 g fresh thalline with NaCl 50 and at the shaking speed of 150 r min-1 during digestion. The protoplast yield can reach 2.62 + 0.09 million per 0.5 g fresh leave under the optimum condition. The enzyme activity is inhibited by Ca2+ and slightly enhanced by Fe2+ and Mn2+ at concentrations of 0.05, 0.08 and 0.10 molL-1. |
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| Keywords: | alginate lyase Undaria pinnatifida protoplast |
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