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Partial Sequence Analysis of Mitochondrial COI Gene of the Chinese Shrimp, Fenneropenaeus Chinensis
作者姓名:GAO Tianxiang  LI Jian  WANG Qingyin  LIU Jinxian The Key Laboratory of Mariculture  Ministry of Education  Ocean University of China  Qingdao  P.R.China Yellow Sea Fisheries Research Institute  Chinese Academy of Fishery Sciences  Qingdao  P.R.China
作者单位:GAO Tianxiang,LI Jian,WANG Qingyin,LIU Jinxian The Key Laboratory of Mariculture,Ministry of Education,Ocean University of China,Qingdao 266003,P.R.China Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,P.R.China
基金项目:国家重点基础研究发展计划(973计划) 
摘    要:Eight hundred and thirty eight base pair fragment of mitochondrial COI gene of wild and cultured populations (CP1, CP4, CP5 and CP6) of Fenneropenaeus chinensis was amplified and sequenced. The A, T, G and C contents of the sequence were 235bp (28.0% ),.307bp (36.6%), 138bp (16.5%) and 158bp (18.9%), respectively. Furthermore, 556bp fragment of the sequence was used to discuss the phylogenetic relationship among 14 Penaeidae species using Alpheus armillatus as the outgroup. From the molecular phylogenetic tree constructed by neighbor-joining method, we obtained three large shrimp groups: Farfantepenaeus, Litopenaeus and Fenneropenaeus group. The results also indicated that there were a closer genetic relationships between F. aztecus and F. paulensis, L. schmitti and L. setiferus, F. indicus and F. merguiensis, and the genus Farfantepenaeus was closer to Litopenaeus.


Partial Sequence Analysis of Mitochondrial COI Gene of the Chinese Shrimp, Fenneropenaeus Chinensis
GAO Tianxiang,LI Jian,WANG Qingyin,LIU Jinxian The Key Laboratory of Mariculture,Ministry of Education,Ocean University of China,Qingdao ,P.R.China Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao ,P.R.China.Partial Sequence Analysis of Mitochondrial COI Gene of the Chinese Shrimp, Fenneropenaeus Chinensis[J].Journal of Ocean University of China,2003,2(2).
Authors:GAO Tianxiang  LI Jian  Wang Qingyin  Liu Jinxian
Abstract:Eight hundred and thirty eight base pair fragment of mitochondrial COI gene of wild and cultured populations (CP1, CP4, CP5 and CP6) of Fenneropenaeus chinensis was amplified and sequenced. The A, T, G and C contents of the sequence were 235bp (28.0% ),.307bp (36.6%), 138bp (16.5%) and 158bp (18.9%), respectively. Furthermore, 556bp fragment of the sequence was used to discuss the phylogenetic relationship among 14 Penaeidae species using Alpheus armillatus as the outgroup. From the molecular phylogenetic tree constructed by neighbor-joining method, we obtained three large shrimp groups: Farfantepenaeus, Litopenaeus and Fenneropenaeus group. The results also indicated that there were a closer genetic relationships between F. aztecus and F. paulensis, L. schmitti and L. setiferus, F. indicus and F. merguiensis, and the genus Farfantepenaeus was closer to Litopenaeus.
Keywords:Fenneropenaeus chinensis  cytochrome oxidase subunit 1(COI)  DNA sequence  Penaeidae  phylogeny
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