Inheritance pattern of microsatellite loci and their use for kinship analysis in the Japanese scallop Patinopecten yessoensis |
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| Authors: | Kefeng Xu Qi Li |
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| Institution: | (1) College of Fisheries, Ocean University of China, Qingdao, 266003, P. R. China |
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| Abstract: | The inheritance mode of seven microsatellite markers was investigated in Patinopecten yessoensis larvae from four controlled crosses, and the feasibility of using these markers for kinship estimation was also examined.
All the seven microsatellite loci were compatible with Mendelian inheritance. Neither sex-linked barriers to transmission
nor major barriers to fertilization between gametes from the parents were evident. Two of the seven loci showed the presence
of null alleles in two families, suggesting the need to conduct comprehensive species-specific inheritance studies for microsatellite
loci used in population genetic studies. However, even if the null allele heterozygotes were considered as homozygotes in
the calculation of genetic distance, offspring from four families were all unambiguously discriminated in the neighbor-joining
dendrogram. This result indicates that the microsatellite markers used may be capable of discriminating between related and
unrelated scallop larvae in the absence of pedigree information, and of investigating the effective number of parents contributing
to the hatchery population of the Japanese scallop. |
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| Keywords: | Patinopecten yessoensis larvae allelic transmission kinship microsatellites |
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