Preliminary evaluation of macrophage pinocytosis as a technique to monitor fish health |
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| Institution: | 1. Departamento de Ciencias de la Tierra y del Medio Ambiente, Universidad de Alicante, Campus San Vicente, San Vicente del Raspeig, 03080 Alicante, Spain;2. Dipartimento di Scienze Pure e Applicate (DiSPeA), Università degli Studi di Urbino Carlo Bo, Campus Scientifico E. Mattei, 61029 Urbino, Italy;3. Departmentul de Geologie, Universitatea “Alexandru Ioan Cuza” din Iași, 20A, Carol I, 700505 Iași, Romania;1. Fish Ecology and Conservation Physiology Laboratory, Department of Biology, Carleton University, Ottawa, ON, Canada;2. Department of Wildlife, Fish and Conservation Biology, University of California-Davis, Davis, CA, United States;3. Department of Ecology, Evolution, and Marine Biology, University of California-Santa Barbara, Santa Barbara, CA, United States;4. Department of Zoology, University of British Columbia, Vancouver, BC, Canada;1. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Departamento de Medio Ambiente, Carretera de la Coruña, Km 7.5, Madrid, Spain;2. Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas (CIEMAT), Avenida. Complutense 22, E-28040 Madrid, Spain;1. Institute of Biomedical Chemistry, Pogodinskaya Street, 10, Build 8, Moscow 119121, Russia;2. Pirogov Russian National Research Medical University, Ostrovitianov Street, 1, Moscow 117997, Russia;3. Institute of bioorganic chemistry NASB, 5 Academician V.F. Kuprevich Street, Build 2, Minsk BY-220141, Belarus |
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| Abstract: | A recently developed spectrophotometric technique was used to examine the effect of environmental pollutants on pinocytosis by fish macrophages. Spot, Leiostomus xanthurus, and hogchoker. Trinectes maculatus, were captured from the York River, a relatively non-polluted ricer, and from the Elizabeth River, known to be highly contaminated with polynuclear aromatic hydrocarbons (PAHs). Kidney cell types were separated by density gradient centrifugation and single cell suspensions of macrophages were used in in vitro studies. The pinocytic activity of kidney macrophages from spot and hogchoker was determined by means of a colorimetric assay of ingested neutral red dye. It was found that the pinocytic activity of macrophages from spot exposed to contaminated waters did not differ from control values. However, there was a two-fold increase in pinocytic activity in pollutant-stressed hogchoker. It is proposed that hogchoker could serve as an indicator species to monitor pollution effects in the environment. |
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