| Cloning, Expression and Characterization of Translationally Controlled Tumor Protein （TCTP） Gene from Flatfish Turbot （Scophthalmus maximus） |
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| 作者单位： | WANG Jian（College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P. R. China） ;
GUO Huarong（College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P. R. China） ;
ZHANG Shicui（College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P. R. China） ;
YIN Licheng（College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P. R. China） ;
GUO Bin（College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P. R. China） ;
WANG Shaojie（College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P. R. China） ; |
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| 基金项目： | 国家重点基础研究发展计划(973计划)，the Student Research Training Program of Ocean University of China |
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| 摘 要： |
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| 关 键 词： | 大比目鱼 大菱鲆 基因克隆 肿瘤 基因表达 |
| 收稿时间： | 21 June 2006 |
Cloning,expression and characterization of translationally controlled tumor protein (TCTP) gene from flatfish turbot (<Emphasis Type="Italic">Scophthalmus maximus</Emphasis>) |
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| Authors: | Jian Wang Huarong Guo Shicui Zhang Licheng Yin Bin Guo Shaojie Wang |
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| Institution: | College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P. R. China |
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| Abstract: | A full-length cDNA encoding translationally controlled tumor protein of marine flatfish turbot (Scophthalmus maximus), SmTCTP, was isolated with rapid amplification of cDNA Ends (RACE). SmTCTP consisted of a 5' untranslated region (UTR) of 84 bp, a 3' UTR of 451 bp and an open reading flame (ORF) of 513 bp, encoding a protein of 170 amino acid residues, which contained two signature sequences of TCTP family. The 5'UTR of SmTCTP started with a 5'-terminal oligopyrimidine tract (5'-TOP), a typical feature for translationaily controlled mRNAs. The deduced amino acid sequence of SmTCTP was similar to the other known verte-brate TCTPs in a range of 58.8% to 64.1%. The length offish TCTPs was diverse among species, e.g., TCTP of turbot and sea perch (Lateolabrax japonicus) is 170 aa in length, while that of zebrafish (Danio rer/o) and rohu (Labeo rohita) is 171 aa in length. North-ern blot analysis revealed that SmTCTP has only one type of mRNA. Its expression level in albino skin was slightly higher than that in normal skin. We constructed the pET3Oa-SmTCTP expression plasmid. The recombinant protein of His-tag SmTCTP was over-expressed in E. coli, purified and identified with peptide mass fingerprinting. These results may pave the way of further inves-tigation of the biological function of TCTP in fish. |
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| Keywords: | translationally controlled tumor protein (TCTP) turbot RACE Northern blot expression peptide mass fingerprinting |
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