| Amylase Production by the Marine Yeast Aureobasidium pullulans N13d |
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| 作者姓名： | LI Haifeng CHI Zhenming * WANG Xiaohong and MA Chunling UNESCO Chinese Center of Marine Biotechnology Ocean University of China Qingdao P. R. China |
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| 作者单位： | LI Haifeng，CHI Zhenming *，WANG Xiaohong，and MA Chunling UNESCO Chinese Center of Marine Biotechnology，Ocean University of China，Qingdao 266003，P. R. China |
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| 摘 要： | 1 Introduction Amylases are enzymes which hydrolyze starch molecules to give diverse products including dextrin and pro-gressively smaller polymers composed of glucose units(Windish et al., 1965; Pandey et al., 2000; Chi et al.2001). Amylase is a kind of very important enzyme andconstitutes a class of industrial enzymes sharing approximately 25% of the enzyme market (Sindhu et al.1997; Rao et al., 1998). Amylases are universally distributed throughout the animal, plant and microbial kingdoms…
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Amylase Production by the Marine Yeast Aureobasidium pullulans N13d |
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| LI Haifeng,CHI Zhenming *,WANG Xiaohong,and MA Chunling UNESCO Chinese Center of Marine Biotechnology,Ocean University of China,Qingdao ,P. R. China.Amylase Production by the Marine Yeast Aureobasidium pullulans N13d[J].Journal of Ocean University of China,2007,6(1):60-65. |
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| Authors: | LI Haifeng CHI Zhenming WANG Xiaohong MA Chunling |
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| Abstract: | The marine yeast strain N13d, producing an extracellular amylase, was isolated from the deep sea sediments of the Pacific Ocean. This strain was identified to be Aureobasidium pullulans by 18S rRNA gene sequence analysis and routine yeast identification methods. The optimal sea water medium for amylase production by this yeast strain was 1.0% peptone and 1.0% soluble starch with pH 4.0. The optimal conditions for amylase production by this yeast strain were with temperature 28 ℃, aeration rate 6Lmin-1 and agitation speed 250 rmin-1. Under these conditions, 58.5 units of amylase activity per mg protein were produced within 56h of fermentation. |
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| Keywords: | extracellular amylase marine yeast Aureobasidium pullulans fermentation |
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