| 哲罗鱼(Hucho taimen)染色体组型与DNA含量分析 |
| |
| 引用本文： | 薛淑群,尹洪滨,尹家胜.哲罗鱼(Hucho taimen)染色体组型与DNA含量分析[J].广东海洋大学学报,2010,30(3):6-10. |
| |
| 作者姓名： | 薛淑群 尹洪滨 尹家胜 |
| |
| 作者单位： | 中国水产科学研究院黑龙江水产研究所,黑龙江,哈尔滨,150070 |
| |
| 基金项目： | 国家科技基础条件平台建设项目，国家科技支撑计划 |
| |
| 摘 要： | 采用体内注射PHA和秋水仙素、肾细胞短期培养、常规空气干燥法制备哲罗鱼(Hucho taimen)的染色体。对其肾细胞染色体数目统计分析表明,哲罗鱼染色体组有84条染色体,核型公式为2n=18m+16sm+34st+16t,其染色体总臂数(NF)为118。采用流式细胞分析仪测定哲罗鱼的DNA含量,与鸡血细胞标准对照的比值为2.23±0.17,以鸡红细胞DNA含量2.30pg·N~(-1)计,则哲罗鱼的体细胞DNA含量为5.12pg·N~(-1)。哲罗鱼染色体数目和DNA含量体现为四倍体特征。
|
| 关 键 词： | 哲罗鱼 染色体 核型 DNA含量 |
Analysis on Chromosome and DNA Content of Hucho taimen |
| |
| XUE Shu-qun,YIN Hong-bin,YIN Jia-sheng.Analysis on Chromosome and DNA Content of Hucho taimen[J].Journal of Zhanjiang Ocean University,2010,30(3):6-10. |
| |
| Authors: | XUE Shu-qun YIN Hong-bin YIN Jia-sheng |
| |
| Abstract: | Metaphase chromosome specimens of Hucho taimen injected with PHA and colchicin,were prepared from short-term culture of kidney cells with air-drying technique.Its diploid chromosome number is 2n = 84.The karyotype is composed of 18m,16sm,34st and 16t chromosomes,NF=118.Content of diploid nucleus DNA was measured from somatic cell using flow cytometer(Beckman cellLab Quant,made in USA),the DNA content(2.30 pg·N~(-1)) of erythrocytes of chick(Gallus sp) as standard.The ratio of DNA content between Hucho taimen and chick is 2.23±0.17,so DNA content of Hucho taimen is 5.12 pg·N~(-1),which correspond with the result of measured chromosome number as ploidy.According to the present results and date reported,Hucho taimen is possibly an tetraploid(4n). |
| |
| Keywords: | Hucho taimen chromosome karyotype DNA content |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
