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Nutrients limiting the algal growth potential (AGP) in the Po River plume and an adjacent area, Northwest Adriatic Sea: Enrichment bioassays with the test algaeNitzschia closterium andThalassiosira pseudonana |
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| Authors: | Serge Y Maestrini Brigitte R Berland Martine Bréret Christian Béchemin Roberto Poletti Attilio Rinaldi |
| Institution: | 1. Centre de Recherche en Ecologie Marine et Aquaculture de L’Houmeau (CNRS-IFREMER), BP 5, 17137, L’Houmeau, France 2. Station Marine d’Endoume Chemin de la Batterie des Lions, Centre d’Océanologie Université Aix-Marseille II, 13007, Marseille, France 3. Centro Ricerche Marine, U.O. Batello Oceanografico “Daphne” Viale Vespucci 2, 47042, Cesenatico, Italy |
| Abstract: | From April 1993 to March 1994, 135 samples were collected at two sites in the coastal Adriatic Sea, both near (14.5 km) and far (63 km) from the Po River delta. The nutrient(s) limiting algal growth potential (AGP) were estimated by bioassay usingNitzshia closterium (local isolate) andThalassiosira pseudonama (strain 3-H). Estimates were also made by comparing the nutrient molar ratios, ΣN:P and ΣN:Si (whereΣN=NO3 ?+NO2 ?+NH4 ++urea), to the Redfield Ratio (16∶1, 1∶1). According to the bioassay results, phosphorus was the sole nutrient limiting AGP in 2% of the samples and was the most limiting nutrient in 69% of the samples; nitrogen was sole limiting nutrient in 18% of the samples. In 11% of samples, nitrogen and phosphorus were equally co-limiting. Omission of phosphorus from spike enrichments allowed, on average, only a 1.6-fold increase in biomass over that in the unenriched controls. Similar omission of nitrogen allowed a 4-fold increase, while silicon, iron, and micronutrients resulted in 14-fold, 18-fold, and >20-fold increases, respectively. In most of the samples, ΣN:P was much greater than 16, indicating a marked phosphorus deficiency, while ΣN:Si values suggested that silicon was the third most limiting nutrient in 35% of samples. In water collected for from the Po delta, the yield ofN. closterium was not limited by any nutrients other than the three major ones: P, N and Si. In these same waters,T. pseudonana was also potentially limited by iron and, to a lesser extent, by vitamins. The role of iron varied. In samples collected near the Po delta, iron acted as the third most limiting nutrient forN. closterium in June and September; it appeared 29 times out of 78 on the list of potentially limiting nutrients forT. pseudonana, including 5 times as the most limiting. Altogether, comparison with published results suggests that the roles of iron and silicon in AGP limitation have increased during the past three decades, and could become even more important if eutrophication in the Adriatic Sea continues to increase. |
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