| 长期超低温保存后真鲷精子的质量变化 |
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| 引用本文： | 陈亚坤,刘清华,赵春彦,肖志忠,徐世宏,马道远,李 军.长期超低温保存后真鲷精子的质量变化[J].海洋科学,2010,34(6):50-54. |
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| 作者姓名： | 陈亚坤 刘清华 赵春彦 肖志忠 徐世宏 马道远 李 军 |
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| 作者单位： | 1. 中国科学院,海洋研究所,山东,青岛,266071;中国科学院,研究生院,北京,100039
2. 中国科学院,海洋研究所,山东,青岛,266071 |
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| 基金项目： | 中国科学院海洋研究所知识创新领域前沿项目(Y02507101Q) |
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| 摘 要： | 通过对精子运动率、受精率和超微结构的观察,研究了2003~2009年分5批冷冻保存的真鲷(Pagrus major)精子经1~73个月保存后质量变化情况。冷冻精子在40℃水浴中解冻,大约100~110 s转化为液态后取出,用海水激活后观察精子运动率,人工受精6~8 h后观察受精率,同时精子用2.5%戊二醛前固定,经处理后用扫描电镜和透射电镜观察精子超微结构。结果表明,冷冻精子的最高运动率(87.67%±2.52%)和受精率(71.33%±8.84%)都是2009年保存1个月的精子,保存1,13,26个月的精子运动率和受精率差异不显著(P0.05),保存48个月后的精子运动率和受精率显著低于保存1个月的精子(P0.05),2003年保存的精子(73个月)运动率(50.67%±5.31%)和受精率最低(39.56%±0.69%)。精子的超微结构损伤主要包括精子头部损伤,线粒体损伤以及精子质膜的损伤。
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| 关 键 词： | 低温保存 真鲷 (Pagrus major) 精子 运动率 受精率 |
| 收稿时间： | 2010/3/22 0:00:00 |
| 修稿时间： | 2010/4/10 0:00:00 |
Quality change of Pagrus major sperm after long-term cryopreservation |
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| CHEN Ya-kun,LIU Qing-hu,ZHAO Chun-yan,XIAO Zhi-zhong,XU Shi-hong,Ma Dao-yuan and LI Jun.Quality change of Pagrus major sperm after long-term cryopreservation[J].Marine Sciences,2010,34(6):50-54. |
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| Authors: | CHEN Ya-kun LIU Qing-hu ZHAO Chun-yan XIAO Zhi-zhong XU Shi-hong Ma Dao-yuan and LI Jun |
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| Abstract: | After cryopreservation (1-73 months), the quality of Pagrus major sperm, which was cryopreserved from 2003 to 2009 in 5 batches, was examined by observing motility, fertilization rate and ultrastructures. The cryopreserved sperm were thawed in 40 oC water bath for 100~110 s. After activation with seawater, the motility of the sperm was estimated; 6-8 h after artificial fertilization, fertilization rate was examined; and after being fixed with by 2.5% glutaraldehyde, the ultrastructure of sperm was studied with scanning electron microscope and transmission electron microscope. The highest motility (87.67%±2.52%) and fertilization rate (71.33%± 8.84%) were obtained in sperm after a 1-month cryopreservation, even though they were not significantly different from those of the sperm after a 13-month or and 26-month cryopreservation (P>0.05). After a 48-month cryopreservation, the motility and fertilization rate decreased significantly in comparison with those of sperm after a 1-month cryopreservation (P<0.05). The lowest motility (50.67% ± 5.31%) and fertilization rate (39.56% ± 0.69%) were obtained in sperm cryopreserved for 73 months; main ultrastructural damage was observed in the head, mitochondria and membranes of sperm. |
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| Keywords: | cryopreservation Pagrus major sperm motility fertilization rate |
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