| The genotoxic effects of benzo[a]pyrene and methamidophos on black porgy evaluated by comet assay |
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| 作者姓名： | 刘日先 洪华生 王新红 王克坚 王春光 |
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| 作者单位： | State Key Laboratory for Marine Environmental Science, Environmental Science Research Center, Xiamen University, Fujian 361005, China |
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| 基金项目： | The work was supported by Natural Science Foundation of China （Grant No. A20077023 and C40106012） ACKN0WLEDGEMENT We thank Dr. Wang Shuhong from Jimei University for her help in courtesy of using experimental facilities. We also thank the graduate students from our laboratory for their helps in sampling. |
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| 摘 要： | In this study, two common pollutants （benzoa]pyrene and methamidophos） in marine environment were tested by comet assay for their inducement of in vivo genotoxic effect to the blood cells of black porgy （ Acanthopagrus schlegeli）. The fish was exposed to 2 μg/L of benzoa]pyrene （BaP） and methamidophos, and their mixture. The assay was performed on whole blood at 2 h, 5 h, 24 h and 96 h exposure intervals. A significant increase in DNA damage was observed in each treatment with the pollutants. Additive effect of BaP and methamidophos was also found in the experiment. However, the decrease ratios of DNA damage for 5 h and 96 h exposure interals compared with 2 h and 24 h exposure ones, respectively, were noticed. This phenomenon may be explained by the function of repairing process via enzyme cytochrome P450 in the animal. Evidence of the genotoxicity of organophosphorus pesticides （OPs） and polynuclear aromatic hydrocarbons （PAHs） on marine fish are discussed in this paper.
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| 关 键 词： | 棘鬣鱼 中毒 DNA 水生生物 环境污染 |
| 收稿时间： | 2004-08-25 |
| 修稿时间： | 2005-05-02 |
The genotoxic effects of benzo[a]pyrene and methamidophos on black porgy evaluated by comet assay |
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| Liu Rixian,Hong Huasheng,Wang Xinhong,Wang Kejian,Wang Chunguang.The genotoxic effects of benzo[a]pyrene and methamidophos on black porgy evaluated by comet assay[J].Chinese Journal of Oceanology and Limnology,2005,23(4):455-460. |
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| Authors: | Liu Rixian Hong Huasheng Wang Xinhong Wang Kejian Wang Chunguang |
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| Institution: | (1) State Key Laboratory for Marine Environmental Science, Environmental Science Research Center, Xiamen University, 361005 Fujian, China |
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| Abstract: | In this study, two common pollutants (benzoa]pyrene and methamidophos) in marine environment were tested by comet assay for
their inducement ofin vivo genotoxic effect to the blood cells of black porgy (Acanthopagrus schlegeli). The fish was exposed to 2 μg/L of benzoa]pyrene (BaP) and methamidophos, and their mixture. The assay was performed on
whole blood at 2 h, 5 h, 24 h and 96 h exposure intervals. A significant increase in DNA damage was observed in each treatment
with the pollutants. Additive effect of BaP and methamidophos was also found in the experiment. However, the decrease ratios
of DNA damage for 5 h and 96 h exposure interals compared with 2 h and 24 h exposure ones, respectively, were noticed. This
phenomenon may be explained by the function of repairing process via enzyme cytochrome P450 in the animal. Evidence of the
genotoxicity of organophosphorus pesticides (OPs) and polynuclear aromatic hydrocarbons (PAHs) on marine fish are discussed
in this paper.
The work was supported by Natural Science Foundation of China (Grant No. A20077023 and C40106012) |
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| Keywords: | black porgy genotoxic effects benzo[a]pyrene methamidophos DNA damage comet assay |
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