| 低温条件下微生物诱导固化对比研究 |
| |
| 引用本文： | 孙潇昊,缪林昌,吴林玉,王呈呈,陈润发.低温条件下微生物诱导固化对比研究[J].岩土力学,2018,39(Z2):224-230. |
| |
| 作者姓名： | 孙潇昊 缪林昌 吴林玉 王呈呈 陈润发 |
| |
| 作者单位： | 东南大学 交通学院岩土工程研究所，江苏 南京 210096 |
| |
| 基金项目： | 国家自然科学基金项目（No.51578147）；江苏省研究生科研与实践创新计划项目（No. KYCX18_0107） |
| |
| 摘 要： | 低温条件下微生物诱导沉淀产率低,制约着微生物诱导固化(MICP)技术的实际工程应用。通过控制不同温度和pH值,对比分析巴氏芽孢杆菌和巨大芽孢杆菌的生长繁殖特征和脲酶活性,同时在胶凝液中添加营养物质和控制尿素浓度和钙离子浓度,研究提高沉淀产率的方法,利用XRD测试分析沉淀晶型。进行渗透性试验和无侧限抗压强度试验,对比分析了不同菌种的砂土固化效果,结果表明,低温条件下巨大芽孢杆菌生长繁殖比巴氏芽孢杆菌快,脲酶活性更高,且巨大芽孢杆菌最适宜p H=8,更适合于碱性环境;可以通过在胶凝液中添加营养物质,控制尿素浓度为1.5 M和醋酸钙浓度为0.5 M增加碳酸钙沉淀产率;低温条件下巨大芽孢杆菌沉淀产率总高于巴氏芽孢杆菌,沉淀晶型为更稳定的方解石;采用巨大芽孢杆菌固化的试样渗透性可降低3～4个数量级,而巴氏芽孢杆菌固化的砂柱渗透性只降低2～3个数量级,其中颗粒粒径越小,渗透性降低越明显,且同等条件下巨大芽孢杆菌固化的砂柱试样强度也大于巴氏芽孢杆菌固化试样。因此,低温条件下巨大芽孢杆菌更适合进行实际工程应用。
|
| 关 键 词： | 低温 巴氏芽孢杆菌 巨大芽孢杆菌 碳酸钙 微生物诱导固化(MICP) |
| 收稿时间： | 2018-06-19 |
Comparative study of microbially induced carbonate precipitation
under low temperature conditions |
| |
| SUN Xiao-hao,MIAO Lin-chang,WU Lin-yu,WANG Cheng-cheng,CHEN Run-fa.Comparative study of microbially induced carbonate precipitation
under low temperature conditions[J].Rock and Soil Mechanics,2018,39(Z2):224-230. |
| |
| Authors: | SUN Xiao-hao MIAO Lin-chang WU Lin-yu WANG Cheng-cheng CHEN Run-fa |
| |
| Institution: | Institute of Geotechnical Engineering, Southeast University, Nanjing, Jiangsu 210096, China |
| |
| Abstract: | At low temperature, deposition rate induced by bacteria is often low, which restricts the practical engineering application of microbial solidification technology. The growth and reproduction characteristics and urease activity of Sporosarcina pasteurii and Bacillus megaterium are analyzed by controlling different temperature and pH value. By adding nutrients to the gelling solution and controlling urea concentration and calcium ion concentration the precipitation yields of different strains is studied, and XRD test is conducted to analyze precipitation crystal type. The solidification effect of different strains is studied by the comparison of permeability and unconfined compressive strength tests. The results show that at low temperature, the growth and reproduction of Bacillus megaterium are faster, and urease activity is higher, and the optimum pH value of Bacillus megaterium is 8, meaning that it is more suitable for alkaline environment. The precipitation yields can be increased by adding nutrients to the gelling solution, increasing concentration of urea to 1.5 M and controlling calcium acetate concentration at 0.5 M. The deposition rate of Bacillus megaterium is always higher than Sporosarcina pasteurii at low temperature, and the precipitated crystal is more stable calcite. The permeability of samples solidified by Bacillus megaterium is reduced by 3 to 4 orders of magnitude, while that with Sporosarcina pasteurii is only 2 to 3 orders of magnitude. The smaller the particle size, lower the permeability. Under the same conditions, the strength of samples solidified with Bacillus megaterium is stronger than ones solidified with Sporosarcina pasteurii. Therefore, Bacillus megaterium is more suitable for practical engineering applications at low temperature. |
| |
| Keywords: | low temperature Sporosarcina pasteurii bacillus megaterium calcium carbonate microbially induced carbonate precipitation(MICP) |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《岩土力学》浏览原始摘要信息 |
| 点击此处可从《岩土力学》下载免费的PDF全文 |
|
