| The preparation of low electroendosmosis agarose and its physico-chemical property |
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| 作者姓名： | 胡如桂 刘效磊 刘力 张全斌 张虹 牛锡珍 |
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| 作者单位： | Institute of Oceanology Chinese Academy of Sciences，Normal School of Infant Education and Especial Education，Institute of Oceanology Chinese Academy of Sciences，Institute of Oceanology Chinese Academy of Sciences，Institute of Oceanology Chinese Academy of Sciences，Institute of Oceanology Chinese Academy of Sciences，Qingdao 266071 China Graduate University of Chinese Academy of Sciences Beijing 100049 China，Weifang 261021 China，Qingdao 266071 China，Qingdao 266071 China，Qingdao 266071 China，Qingdao 266071 China |
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| 摘 要： | Studies on Gelidium amansii agar fractionations were carried out in this paper. Gelidium amansii agar was fractionated on DEAE-Cellulose, and four fractions were obtained sequentially. The fractions were analyzed on physical and chemical properties, and IR and 13C-NMR spectroscopy applied for elucidating the chemical structure. Among the four fractions obtained, water fraction measured up to the standard of low EEO agarose. The sulfate content, ash content, electroendosmosis and gel strength (1%) of water fraction were 0.16%, 0.34%, 0.12 and 1 130g/cm2 respectively, similar to those of the Sigma products.
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| 关 键 词： | 内电渗 琼脂糖 纤维素 物理成分 |
| 收稿时间： | 26 May 2006 |
| 修稿时间： | 2006-05-26 |
The preparation of low electroendosmosis agarose and its physico-chemical property |
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| Hu?Rugui,Liu?Xiaolei,Liu?Li,Zhang?Quanbin,Zhang?Hong,Niu?Xizhen.The preparation of low electroendosmosis agarose and its physico-chemical property[J].Chinese Journal of Oceanology and Limnology,2007,25(4):367-372. |
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| Authors: | Hu Rugui Liu Xiaolei Liu Li Zhang Quanbin Zhang Hong Niu Xizhen |
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| Institution: | Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Graduate University of Chmese Academy ofSctences, Beijing 100049, China;Normal School of lnfant Education and Especial Education, Weifang 261021, China |
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| Abstract: | Studies on Gelidium amansii agar fractionations were carried out in this paper. Gelidium amansii agar was fractionated on DEAE-Cellulose, and four fractions were obtained sequentially. The fractions were analyzed on physical and chemical properties, and IR and 13C-NMR spectroscopy applied for elucidating the chemical structure. Among the four fractions obtained, water fraction measured up to the standard of low EEO agarose. The sulfate content, ash content, electroendosmosis and gel strength (1%) of water fraction were 0.16%, 0.34%, 0.12 and 1 130g/cm2 respectively, similar to those of the Sigma products. |
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| Keywords: | DEAE-Cellulose agarose electroendosmosis (EEO) |
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