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Comparative study on the allergenicity of different Litopenaeus vannamei extract solutions |
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| Authors: | Lisha Wu Haixin Lin Guoying Wang Zongchao Lu Guanzhi Chen Hong Lin Zhenxing Li |
| Institution: | 1. Food Safety Laboratory, College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, P. R. China 2. Departments of Immunology, Qingdao University, Qingdao, 266383, P. R. China 3. The Affiliated Hospital of Medical College, Qingdao University, Qingdao, 266003, P. R. China |
| Abstract: | Allergen extracts are widely used for allergy diagnosis and treatment. The application of shrimp extract is hampered due to the low protein concentration and the inconsistent allergenicity. Extracting solutions are considered to be the primary limiting factor of protein extraction from crustaceans. This study aimed to select an optimal solution for shrimp protein extraction by comparing the allergenicity of different shrimp extracts. The effect of 7 existing or modified extracting solutions were evaluated, including the glycerol-NaCl solution, the glycerol Cocaine's solution, the buffered saline solution, the Cocaine's solution, the Glucose leaching solution, 1 mol L-1 KCl solution, and 0.01 mol L-1 phosphate buffered saline solution with and without dithiothreitolor(DTT). The quantitative(protein concentration) and qualitative parameters(SDS-PAGE protein patterns and immuno-reactivity) were determined using the sodium dodecyl sulfate polyacrylamide gel electrophoresis, enzyme linked immunosorbent assay and immunoblotting assay. Results showed that the 1 mol L-1 KCl solution with DTT was optimal for shrimp protein extraction, which yielded high concentration and allergenicity in the protein extract, including major and minor allergens. The 1 mol L-1 KCl solution with DDT is proposed for preparation of shrimp extract and associated allergy diagnosis, as well as potential applications for other crustaceans. |
| Keywords: | shrimp allergen protein extract extracting solution allergenicity |
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