Living ostracod and hydrochemical samples were collected synchronously from more than 50 lakes and small water body in the eastern edge area of the Tibetan Plateau, Northern Tibetan, Southern Tibet and mid-Tibet. The comparison of the adult body length ofLimnocythere inopinata and hydrochemical parameters of their habitats shows that a quantitative relationship exists between the adult body length and salinity expressed as conductivity. An empirical formula to reconstruct paleosalinity is suggested first and applied to salinity reconstruction of CE-2 core from Cuoe Lake, Tibet. The method is verified by comparing its outcome with results of other environment reconstruction methods.