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Iron ions increase the thermostability of phycocyanin ofSpirulina maxima
Authors:Jian-hong  Li  Zi-hou  Tai  Tseng  Chao-Tsi
Institution:1.Department of Biology, Nanjing Normal University, 210097, Nanjing, China
;2.State Key Laboratory of Coordination Chemistry, Nanjing University, 210093, Nanjing, China
;3.Department of Biological Science and Biotechnology, Nanjing University, 210093, Nanjing, China
;
Abstract:

A spectral method to investigate the effect of Fe3+, Fe2+ on the thermostability of phycocyanin (PC) ofSpirulina maxima showed that iron ions provent decrease of visible light absorbance and fluorescence intensity of PC. Increase in denaturation temperature caused by Fe3+ was observed by the micro-differential scanning calorimetric method. All results showed iron ions maintain the aggregation stability of the PC. The absorption spectrum of phycocyanobilin (PCB, a prosthetic group of PC) with Fe3+ in chloroform was quite different from that of free PCB.

Keywords:
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