| 带形蜈蚣藻多糖和可溶性蛋白含量测定及藻红蛋白分析鉴定 |
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| 引用本文： | 牛建峰,王广策,曾呈奎,薛志欣.带形蜈蚣藻多糖和可溶性蛋白含量测定及藻红蛋白分析鉴定[J].海洋科学,2006,30(8):50-53. |
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| 作者姓名： | 牛建峰 王广策 曾呈奎 薛志欣 |
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| 作者单位： | 1. 中国科学院,海洋研究所,山东,青岛,266071;中国科学院,研究生院,北京,100039
2. 中国科学院,海洋研究所,山东,青岛,266071 |
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| 摘 要： | 用沸水浴的方法提取了带形蜈蚣藻(Grateloupia turuturuYamada)中的多糖成分,并用“苯酚-硫酸法”测定了其含量。用此法得到的多糖标准曲线,相关系数r=0.998 4,结果显示带形蜈蚣藻多糖质量分数为44.91%;利用Bradford法,测得60℃恒温干燥的带形蜈蚣藻可溶性总蛋白质量分数为0.33%;用简单的羟基磷灰石柱层析的方法分离了藻红蛋白,光谱检测证明为R-藻红蛋白,其在带形蜈蚣藻中的质量分数为0.114 5%。本实验测定方法可靠且简便易行。
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| 关 键 词： | 带形蜈蚣藻(Grateloupia turuturu Yamada) 多糖 分光光度法 Bradford法 |
| 文章编号： | 1000-3096（2006）08-0050-04 |
| 收稿时间： | 2004-10-10 |
| 修稿时间： | 2005-01-05 |
Determination of amylose and dissolvable protein in Grateloupia turuturu Yamada by spectrophotometry identifying of its phycoerythrin |
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| NIU Jian-feng,WANG Guang-ce,Tseng C.K,XUE Zhi-xin.Determination of amylose and dissolvable protein in Grateloupia turuturu Yamada by spectrophotometry identifying of its phycoerythrin[J].Marine Sciences,2006,30(8):50-53. |
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| Authors: | NIU Jian-feng WANG Guang-ce Tseng CK XUE Zhi-xin |
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| Institution: | 1. Institute of Oceanology, the Chinese Academy of Sciences, Qingdao 266071, China;2. Graduate School, the Chinese Academy of Sciences, Beijing 100039, China |
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| Abstract: | Using the boiling water is to extract amylase from Grateloupia turuturu Yamada,and then,spectrophotometry was selected to determine the content of amylases.We got a standard curve and its correlation coefficient was r=0.998 4.Results presented that the content of amylases in G.turuturu Yamada was(44.91%).Bradford was selected to determine the content of dissolvable protein in G.turuturu Yamada. It was 0.33%.Hydroxyapatite chromatography was exploded to purify Phycoerythrin from extraction.The Absorption Spectra showed it was R-phycoerythrin.The content of R-Phycoerythrin in G.turuturu Yamada was(0.114 5%).The methods were proved to be simple,precise and feasible. |
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| Keywords: | Grateloupia turuturu Yamada amylase spectrophotometry Bradford |
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