| Molecular identification of Prorocentrum （Dinophyceae） species |
| |
| 引用本文： | 张诚 韩笑天 邹景忠 俞志明 宋秀贤 ChristianSchuett.Molecular identification of Prorocentrum （Dinophyceae） species[J].中国海洋与湖沼学报,2005,23(3):317-322. |
| |
| 作者姓名： | 张诚 韩笑天 邹景忠 俞志明 宋秀贤 ChristianSchuett |
| |
| 作者单位： | [1]InstituteofOceanology,ChineseAcademyofSciences,Qingdao266071,China [2]BiologischeAnstaltHelgoland,Helgoland,D-27483,Germany |
| |
| 基金项目： | Project supported by National Basic Research Priorities Program (2001CB409701, 2001CB409710) and supported by NSFC (40376040, 40025614)* Project supported by National Basic Research Priorities Program (2001CB409701, 2001CB409710) and supported by NSFC (40376040, 40025614) |
| |
| 摘 要： | A fragment of a large sub-unit ribosomal DNA (LrDNA) of 12 strains of Prorocentrum species was amplified by polymerase chain reaction (PCR). The PCR products were digested by 3 restriction endonucleases (Cfo Ⅰ, Hae Ⅲ, and RSA Ⅰ) and then resolved in agarose gels. Results show that different species had different RFLP patterns, except for P arcuatum (ME 131), which had the same pattern to P. micans (ME 160 and 04).The same fragment of 19 strains of the genus was also amplified and subjected to denaturing gradient gel electrophoresis (DGGE). 11 different patterns were resolved. Different cultures of a same species had the same pattern. The results of RFLP and DGGE analyses showed that eight newly isolated epibenthic Prorocentrum species were different from each other, and also from other cultured ones examined in this study. P arcuatum(MEI32) could not be differentiated from P. micans (MEI60 and 04), it was probably mis-identified, since they are quite different morphologically. P. redfieldii (MEI38) could also not be distinguished form P. triestinium(MEI32), it should be regarded as a synonym ofP. triestinium. Unexpectedly, a restriction site was found in P.micans, compared with previous sequence data.
|
| 关 键 词： | 浮游生物 分子生物学 DNA 基因工程 |
Molecular identification of Prorocentrum (Dinophyceae) species |
| |
| ZHANG Cheng,HAN Xiaotian,ZOU Jingzhong,YU Zhiming,SONG Xiuxian,Christian Schuett.Molecular identification of Prorocentrum (Dinophyceae) species[J].Chinese Journal of Oceanology and Limnology,2005,23(3):317-322. |
| |
| Authors: | ZHANG Cheng HAN Xiaotian ZOU Jingzhong YU Zhiming SONG Xiuxian Christian Schuett |
| |
| Abstract: | A fragment of a large sub-unit ribosomal DNA (LrDNA) of 12 strains of Prorocentrum species was amplified by polymerase chain reaction (PCR). The PCR products were digested by 3 restriction endonucleases (Cfo I, Hae III, and RSA I) and then resolved in agarose gels. Results show that different species had different RFLP patterns, except for P. arcuatum (ME 131), which had the same pattern to P. micans (ME160 and 04). The same fragment of 19 strains of the genus was also amplified and subjected to denaturing gradient gel electropho- resis (DGGE). 11 different patterns were resolved. Different cultures of a same species had the same pattern. The results of RFLP and DGGE analyses showed that eight newly isolated epibenthic Prorocentrum species were different from each other, and also from other cultured ones examined in this study. P arcuatum (ME132) could not be differentiated from P. micans (ME160 and 04), it was probably mis-identified, since they are quite differ- ent morphologically. P. redfieldii (ME138) could also not be distinguished form P. triestinium (ME132), it should be regarded as a synonym of P. triestinium. Unexpectedly, a restriction site was found in P. micans, compared with previous sequence data. |
| |
| Keywords: | Prorocentrum LrDNA RFLP DGGE |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
