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Effects of okadaic acid on haemocytes from Mytilus galloprovincialis: A comparison between field and laboratory studies
Institution:1. Jiangsu Engineering Laboratory for Breeding of Special Aquatic Organisms, Huai''an, China;2. Huai''an Fishery Technology Guidance Station, Huai''an, China;1. Department of Environmental Chemistry, IDAEA-CSIC, Jordi Girona 18, 08034 Barcelona, Spain;2. CESAM & Departamento de Biologia, Universidade de Aveiro, 3810-193 Aveiro, Portugal;3. Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of Russian Academy of Sciences (SIPPB SB RAS), Lermontov Str. 132, 664033, Irkutsk, Russia;4. Department of Bioanalytical Ecotoxicology, UFZ — Helmholtz Centre for Environmental Research, Permoserstr. 15, D-04318 Leipzig, Germany;5. Institute of Hydrobiology, Dresden University of Technology, D-01062 Dresden, Germany
Abstract:Individuals of Mytilus galloprovincialis, contaminated with Diarrheic Shellfish Poisoning (DSP) toxins, were studied with the aim to correlate the okadaic acid (OA) body burden and the percentage of damaged haemocytes by quantifying annexin V positive cells by flow cytometry. Results showed less percentage of damaged haemocytes in high OA contaminated samples. These data were compared with results of in vitro assays of mussel haemocytes exposed to increased concentrations of OA. Similarly, haemocytes exposed to the most concentrated OA solution were less damaged.
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