| 毫克级微藻样品中脂肪酸的分离及测定 |
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| 引用本文： | 彭兴跃,王大志.毫克级微藻样品中脂肪酸的分离及测定[J].台湾海峡,1998,17(3):289-293. |
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| 作者姓名： | 彭兴跃 王大志 |
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| 作者单位： | 厦门大学生物系!厦门，361005(彭兴跃，王大志，林晓生，程兆第)，厦门大学国家教委海洋生态环境开放研究实验室!厦门，361005(徐立) |
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| 基金项目： | 厦门大学国家教委海洋生态环境开放研究实验室资助!MEE9706，国家自然科学基金!49306067 |
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| 摘 要： | 针对微藻样品颗粒小的特点，简化了微藻脂肪酸测定的步骤，使脂肪酸在分离的中间损失的可能性减小，有利于毫克级少量微藻样品的测定，对几种微藻样品的实测结果表明：即使１ｍｇ左右的微藻样吕也可有效地测出各饱和及不饱和的组份。本测定方法 为其它种类微量样品的脂肪酸测定提供参考。
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| 关 键 词： | 微藻 脂肪酸 气相色谱 分离 测定 微藻样品 |
Fast separation and measurement of fatty acids in microalgae sample in milligram |
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| Peng Xingyue, Wang Dazhi,Xu Li, Lin Xiaosheng, and Cheng Zhaodi.Fast separation and measurement of fatty acids in microalgae sample in milligram[J].Journal of Oceanography In Taiwan Strait,1998,17(3):289-293. |
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| Authors: | Peng Xingyue Wang Dazhi Xu Li Lin Xiaosheng and Cheng Zhaodi |
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| Abstract: | This paper describes an analytical method which is more convenient and can fix quantityof fatty acids in a microalgae sample of only about l mg. After freezing dry,the microalgaesample is very loose and has a large specific surface area. Saponification so can be unitedwith extraction and only 2 cm3 solvent is good enough for te first process. There are someother simp1ifications or modifications in our method to make the pretreatment more efficien-cy. Some samples (1 ~ 10mg) of microalgae, including P. tricornutum, D. salina, Chlorellasp.,S. plantensis,are used to test the rationality. The contents of fatty acids in a sample of lmg (S. plantensi ), such as have been determined successfully. |
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| Keywords: | Microalgae fatty acids GC |
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