The study on highly expressed proteins as a function of an elevated ultraviolet radiation in the copepod, Tigriopus japonicus |
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| Authors: | Igor Z Zubrzycki Seunghan Lee Kanghyun Lee Magdalena Wiacek Wonchoel Lee |
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| Institution: | 1. Department of Life Science, College of Natural Sciences, Hanyang University, Seoul, 133-791, Korea
2. Marine Ecosystem Research Division, KIOST, Ansan P.O. Box 29, Seoul, 425-600, Korea
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| Abstract: | The objective of the study was to analyze constantlyhighly expressed proteins as a function of elevated midultraviolet (UVB, 280?C315 nm) radiation in Tigriopus japonicus sensu lato (T. japonicus s.l). We also analyzed associations between kinetics of radiation avoidance, measured as a covered distance per time unit, and highly expressed proteins. The obtained results indicate an increase in T. japonicus s.l. mobility between the control (no radiation) and mild UV radiation levels (15 kJ·m?2). Two-dimensional gel electrophoresis combined with MALDI-MS-MS resulted in 2D protein map comprising of 686 protein spots, of which 19 were identified as highly expressed proteins across all experimental conditions. Obtained results indicate that calpain, vitellogenin, and collagenase are housekeeping protein that are expressed at a constant level independently of environmental changes and that adoption of a locomotive system for the avoidance of a UV source may be, at least partially, supported by hepatopancreas-driven metabolism. |
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