Three-dimensional fluorescence characteristics of dissolved organic matter produced by <Emphasis Type="Italic">Prorocentrum donghaiense</Emphasis> Lu |
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| Authors: | Weihong Zhao Jiangtao Wang Meimei Chen |
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| Institution: | [1]Key Laboratory of Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China [2]Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100. China |
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| Abstract: | Filtration and cross-flow ultrafiltration techniques were used to separate culture media of Prorocentrum donghaiense at the exponential growth, stationary and decline stages into <0.45 μm filtrate, 100 kDa-0.45 μm, 10–100 kDa and 1–10 kDa
retentate and <1 kDa ultrafiltrate fractions. The fluorescence properties of different molecular weights of dissolved organic
matter (DOM) were measured by excitation-emission matrix spectra. Protein-like and humic-like fluorophores were observed in
the DOM produced by P. donghaiense. The central positions of protein-like fluorophores showed a red shift with prolonged growth duration, shifting from tyrosine-like
properties at the exponential growth stage to tryptophan-like properties at the stationary and decline stages. The excitation
wavelengths of protein-like fluorophores exhibited some change in the exponential growth and stationary stages with increased
molecular size, but showed little change in the decline stage. However, the emission wavelengths in the decline stage exhibited
a blue shift. Very distinct C type and A type peaks in humic-like fluorophores were observed. With a prolonged culture time,
the intensities of both of the peaks became strong and the excitation wavelengths of peak A showed a red shift, while the
A:C ratios fell. More than 94% of fluorescent DOM was in the lower than 1 kDa molecular weight fraction. |
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| Keywords: | dissolved organic matter fluorescence excitation-emission matrix spectrum Prorocentrum donghaiense Lu |
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