Vergleichende Untersuchungen über das Phosphataufnahmevermögen ausgewählter Bakterienarten |
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| Authors: | I Rske D Luther H-C Abendroth |
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| Institution: | I. Röske,D. Luther,H.-C. Abendroth |
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| Abstract: | The P-uptake by Acinetobacter calcoaceticus, Pseudomonas aeruginosa and Escherichia coli is determined in batch culture with peptone/glucose/sodium chloride or peptone/acetate/sodium chloride as substrate at 60 to 120 mg/1 orthophosphate for 20 h with cell densities of 2 · 107/ml (Acinetobacter) or 1.2 · 109. The measurements were carried out by means of an Na2HPO4 (32P) addition of 95 to 420 kBq. During the stationary phase the bacteria achieved the following P-contents in the biomass in fg/g bacterium: Acinetobacter 6 to 13, Pseudomonas 0.2 to 0.6, Escherichia 0.04 to 0.09; during the phase of growth Acinetobacter achieved 40 to 100 fg/bacterium. Acetate as the substrate did not result in any increase of the P-uptake. The maximum accumulation with Acinetobacter was 13 % P in the dry substance. |
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