| 三疣梭子蟹养殖群体同工酶的组织特异性及生化遗传分析 |
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| 引用本文： | 王国良,金珊,李政,陈寅儿.三疣梭子蟹养殖群体同工酶的组织特异性及生化遗传分析[J].台湾海峡,2005,24(4):474-480. |
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| 作者姓名： | 王国良 金珊 李政 陈寅儿 |
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| 作者单位： | 宁波大学生命科学与生物工程学院,浙江,宁波,315211 |
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| 基金项目： | 浙江省科技厅资助项目(2003C32032)
浙江省自然科学基金资助项目(302440) |
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| 摘 要： | 应用垂直平板聚丙烯酰胺凝胶电泳技术(PAGE),研究分析了取自浙江舟山朱 家尖养殖区的三疣梭子蟹5种组织中10种同工酶以及肌浆蛋白的表达情况,并进行 生化遗传分析．结果显示,在对40只梭子蟹所检测出的10种同工酶的25个基因座 位中,ADH、ACP-1、CAT-2、EST-2、FDH-2和SOD共6个基因座位为多态,其多态座 位比例(P)为24％,平均杂合度期望值(H)为0．106 6．同时在CAT、LDH、ACP、FDH 4种酶及肌浆蛋白(Pm)中表现出显著的组织特异性．
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| 关 键 词： | 生物化学 三疣梭子蟹 同工酶 组织特异性 生化遗传分析 |
| 文章编号： | 1000-8160（2005）04-0474-07 |
| 收稿时间： | 2004-12-27 |
| 修稿时间： | 2004-12-27 |
Tissue specificity and biochemical genetic analysis of isozyme on cultured Portunus trituberculatus stock |
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| WANG Guo-liang,JIN Shan,LI Zheng,CHEN Yin-er.Tissue specificity and biochemical genetic analysis of isozyme on cultured Portunus trituberculatus stock[J].Journal of Oceanography In Taiwan Strait,2005,24(4):474-480. |
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| Authors: | WANG Guo-liang JIN Shan LI Zheng CHEN Yin-er |
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| Institution: | Life Science and Technology Faculty, Ningbo University, Ningbo 315211, China |
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| Abstract: | Portunus trituberculatu samples were collected from the cultural net in zhoushan, Zhejiang Province. Polyarylamide gel electrophoresis was used to detect the expression of isozymes in 5 tissues of 40 crabs: gill, liver , heart, hemocytoblast ,muscle. 10 isozymes ( ADH, ACP, CAT, ATP,EST,FDH,GDH,LDH,MDH,SOD) and Pm were analyzed and the biochemical genetic results showed that 10 isozymes were coded by 25 gene loci ,6 of which (ADH ,ACP-1,CAT-2,EST-2, FDH-2,SOD) were found polymorphic. The mean proportion of polymorphic loci was 24 % ,and the average heterozygosity per locus was 0. 106 6. Significant tissue specificity was observed in 4 isozymes (CAT, LDH, ACP, FDH ) and Pm. |
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| Keywords: | biochemistry Portunus trituberculatu isozyme tissue specificity biochemical genetic analysis |
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