| 荧光法测定微藻中色氨酸的含量 |
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| 引用本文： | 吴芳,郭卫东,张润,夏恩琴,周宽波.荧光法测定微藻中色氨酸的含量[J].海洋科学,2005,29(10):1-4. |
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| 作者姓名： | 吴芳 郭卫东 张润 夏恩琴 周宽波 |
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| 作者单位： | 厦门大学,海洋系,亚热带海洋研究所,福建,厦门,361005 |
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| 基金项目： | 国家自然科学基金资助项目（40106007） |
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| 摘 要： | 建立了微藻中色氨酸的荧光分析方法。以NaOH为水解液水解微藻，在激发波长225nm，发射波长350nm处测定色氨酸的荧光强度。方法线性范围0～0．07mg／L，检测限为0．003mg／L，回收率90％～100．1％。此法快速、简便、灵敏度高。
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| 关 键 词： | 色氨酸 微藻 荧光法 |
| 文章编号： | 1000-3096（2005）10-0001-04 |
| 收稿时间： | 2004-11-24 |
| 修稿时间： | 2005-01-25 |
Fluorescence measurement of tryptophan content in microalgae |
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| WU Fang,GUO Wei-dong,ZHANG Run,XIA En-qin,ZHOU Kuan-bo.Fluorescence measurement of tryptophan content in microalgae[J].Marine Sciences,2005,29(10):1-4. |
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| Authors: | WU Fang GUO Wei-dong ZHANG Run XIA En-qin ZHOU Kuan-bo |
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| Institution: | Department of Oceanography, Xiamen University, Xiamen 361005, China |
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| Abstract: | This paper set up a procedure of fluorescence method to measure tryptophan in microalgae. NaOH containing 0.5 % soluble starch was used as hydrolytic solution. The fluorescence intensity of tryptophan was determined with excitation wavelength 225 nm and emission wavelength 350 nm. The linear range of calibration graph was 0-0.07 mg/L. The detection limit was 0. 003 mg/L. The recovery rate of sample was 90%-100.1%. |
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| Keywords: | tryptophan microalgae fluorescence method |
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