| 南极石油烃降解嗜冷菌的筛选及其降解特性的研究 |
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| 引用本文： | 郑洲,金青,缪锦来,刘芳明.南极石油烃降解嗜冷菌的筛选及其降解特性的研究[J].海洋通报(英文版),2008,10(2). |
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| 作者姓名： | 郑洲 金青 缪锦来 刘芳明 |
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| 作者单位： | 1. 国家海洋局第一海洋研究所,山东,青岛,266061;海洋生物活性物质国家海洋局重点实验室,山东,青岛,266061
2. 青岛科技大学,山东,青岛,266042 |
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| 摘 要： | 从385株南极海洋细菌中筛选出2株石油烃降解菌，并对其降解特性进行了初步研究。以柴油为唯一碳源进行降解实验的结果表明，南极嗜冷菌NJ276和NJ341在5℃、20d内对柴油的降解率分别达到23．47％和32．15％，在15℃、20d内降解率分别达到43．95％和62．47％，其降解能力随着培养温度的升高而显著增强；石油烃降解残油组分的GC～MS分析表明，柴油经过NJ276降解后的残油组分中能检测到C15～C21七种烷烃，柴油经过NJ341降解后的残油组分只能检测到少量C16,C17和C18三种烷烃。对它们进行16S rDNA基因序列的同源性和系统发育分析表明，菌株NJ276属于假交替单胞菌属（Pseudoalteromonas），NJ341属于科尔韦尔氏属（Colwellia）。
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| 关 键 词： | 南极嗜冷菌 石油烃 生物降解 降解特性 |
Antarctic Psychrophile Bacteria Screening for Oil Degradation and Their Degrading Characteristics |
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| ZHENG Zhou,JIN Qing,MIAO Jinlai,LIU Fangming.Antarctic Psychrophile Bacteria Screening for Oil Degradation and Their Degrading Characteristics[J].Marina Science Bulletin,2008,10(2). |
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| Authors: | ZHENG Zhou JIN Qing MIAO Jinlai LIU Fangming |
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| Abstract: | Two hydrocarbon degrading bacteria NJ276 and NJ341 were screened from 385 Antarctic marine bacteria and their degrading characteristics were studied.Diesel oil as sole carbon source was used in this study.The results showed that the oil degradation rates of Antarctic psychrophile bacteria NJ276 and NJ341 were 23.47 % and 32.15 %,respectively,after 20 days culturation at 5 °C,and the rates were 43.95 % and 62.47 % respectively after 20 days culturation at 15 °C.The oil degradation abilities were enhanced remarkably with the increasing culture temperature.GC-MSs indicated the residual oil contained C15-C21 7 alkyls after degradation by NJ276,and C16,C17 and C18 3 alkyls after degradation by NJ341.The 16S rDNA gene sequences homology and phylogenetic analysis of the two Antarctic psychrophile bacteria showed that NJ276 belonged to the described genus Pseudoalteromonas and NJ341 belonged to the genus Colwellia. |
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| Keywords: | Antarctic psychrophile bacteria Hydrocarbon Degradation |
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