| 印度洋深海沉积物可培养放线菌分离样品预处理条件的优化 |
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| 引用本文： | 姜钊,张卫花.印度洋深海沉积物可培养放线菌分离样品预处理条件的优化[J].海洋科学进展,2022,40(1):134-144. |
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| 作者姓名： | 姜钊 张卫花 |
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| 作者单位： | 藏药检测技术教育部工程研究中心;西藏民族大学西藏高原相关疾病分子遗传机制与干预研究省级重点实验室 |
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| 基金项目： | 西藏自治区自然科学基金——基于组学技术探讨双歧杆菌属系统分类与糖代谢机制(XZ202001ZR0018G);西藏民族大学校内科研项目——世居高原不同海拔地区的藏族大学生低原反应情况调查研究(19MDQ01);西藏自治区科技创新基地自主研究项目——西藏不同海拔人群肠道微生物宏基因组学研究(XZ2021JR0003G)。 |
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| 摘 要： | 海洋沉积物微生物资源丰富,放线菌是其中的优势类群之一,但是海洋放线菌纯培养难度较大.本文利用响应面法对采自印度洋的深海沉积物样品进行湿热处理条件优化,实验中选取了5个因素设计实验,生长的放线菌经纯化培养后,针对16S rRNA基因进行测序分析,以每种优化条件下培养出的放线菌种类数为响应值,优化结果最终为:湿热处理温度5...
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| 关 键 词： | 印度洋 深海沉积物 细菌分离 响应面优化 |
Optimization of Cultivable Actinomyces Isolated Conditions From Indian Ocean Deep-Sea Sediment |
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| JIANG Zhao,ZHANG Wei-hua.Optimization of Cultivable Actinomyces Isolated Conditions From Indian Ocean Deep-Sea Sediment[J].Advances in Marine Science,2022,40(1):134-144. |
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| Authors: | JIANG Zhao ZHANG Wei-hua |
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| Institution: | (Engineering Research Center of Tibetan Medicine Detection Technology, Ministry of Education, Xianyang 712082, China;Key Laboratory for Molecular Genetic Mechanisms and Intervention Research on High Altitude Disease of Tibet Autonomous Region, Xizang Minzu University, Xianyang 712082, China) |
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| Abstract: | Marine sediments are rich in microbial resources,in which the phylum Actinobacteria is the dominant group.However,it’s difficult to get pure culture of these marine Actinobacteria.In this study five experimental factors were selected to optimize bacteria separation conditions by response surface methodology for deep-sea sediment sample collected from Indian Ocean.The numbers of cultivable Actinobacteria were taken as the response values for optimization test after sequencing the 16S rRNA gene of isolated strains.The results of optimal conditions were bellow:wet and heat treatment temperature 55℃for 1.2727 min,air drying time 1.6970 days,inhibitor concentration 0.9697 times and sea salt 2.1212%.A large number of Actnobacteria strains and potential strains were isolated and cultivated using reselected four culture media based on optimized conditions.Accordingly,isolating methods and isolation media are the crucial factors to recover the microorganisms inhabiting in the sediment environments.This study provides a reference for the optimization of pure culture conditions of microorganisms. |
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| Keywords: | India Ocean deep sea sediments bacteria separation response surface optimization |
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