| 龙须菜匍匐体类愈伤组织诱导及机制分析 |
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| 引用本文： | 樊扬,李纫芷.龙须菜匍匐体类愈伤组织诱导及机制分析[J].海洋与湖沼,2000,31(1):29-34. |
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| 作者姓名： | 樊扬 李纫芷 |
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| 作者单位： | 中国科学院海洋研究所 青岛 266071 |
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| 基金项目： | 山东省自然科学基金!2914 |
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| 摘 要： | 采用在人工模拟潮间带生态环境中养殖多年的龙须菜藻体，经７０％乙醇、ａＣｌＯ或低光等逆境胁迫后，培养新生直立枝，研究匍匐体类愈伤组织的诱导及机制。结果表明，刷落２－４ｍｍ长的新生直立枝培养３０天后，基部出现由丛生太体组成的类愈伤组织。丝状体能够固着、分化，不断分裂形成匍匐体。这是除孢子外，首次从江蓠体细胞获得有固着能力的再生苗。该再生苗弥补了营养枝繁殖中须人工固着的缺点。ＥＬＩＳＡ测定内源激素含量结
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| 关 键 词： | 龙须菜 类愈伤组织 铺匐体 内源激素 诱导 |
| 收稿时间： | 1997/8/10 0:00:00 |
| 修稿时间： | 1998/8/26 0:00:00 |
INDUCTION OF CALLUS-LIKE TISSUE FROM CREEPING TISSUE OF GRACILARIA LEMANIFORMIS (RHODOPHYTA) AND MECHANISM ANALYSIS |
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| FAN Yang and LI Ren-zhi.INDUCTION OF CALLUS-LIKE TISSUE FROM CREEPING TISSUE OF GRACILARIA LEMANIFORMIS (RHODOPHYTA) AND MECHANISM ANALYSIS[J].Oceanologia Et Limnologia Sinica,2000,31(1):29-34. |
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| Authors: | FAN Yang and LI Ren-zhi |
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| Institution: | Institute of Oceanology, The Chinese Academy of Sciences, Qingdao, 266071;Institute of Oceanology, The Chinese Academy of Sciences, Qingdao, 266071 |
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| Abstract: | Gracilaria lemaniformis has been cultured in a tank simulating the intertidal environment for fore years. After the alga with erect thalli and creeping tissue was treated with three kinds of stress: 70% ethanol for 15s, 1% NaClO for 15min and low light intensity, regenerated erect thalli (2-4mm) were separated from creeping tissue and cultured for 30d. Callus-like tissue composed of tufted filaments appeared from the bottom of these thalli. These filaments were able to attach, differentiate, divide, and finally grow into creeping tissue. The obtained seedlings with creeping tissue from callus-like tissue of G. lemaniformis, compared with sporeling, have characteristics of fast growth and anti-epiphyte. This is a new method to resolve seedling resolute of Grcilaria culturation. The results of ELISA show that the in vivo hormone level of erect thalli and creeping tissue changed in response to stress. Especially, the increase of ABA was just the same as higher plants. In conclusion, it is possible that the forming of callus-like tissue and its differentiation are related to the in vivo hormone level. |
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| Keywords: | Gracilaria lemaniformis Callus-like tissue Creeping tissue Hormone Stress |
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