Effects of carboxymethyl-chitosan on wound healing in vivo and in vitro |
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| Authors: | Sikai Peng Wanshun Liu Baoqin Han Jing Chang Minyu Li Xuan Zhi |
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| Institution: | College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P. R. China |
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| Abstract: | Effects of carboxymethyl-chitosan with different molecular weights on wound healing were investigated in vivo and in vitro. A second degree burn model was performed in rats and the accelerative effects of carboxymethyl-chitosan on wound repair
were observed. Contents of transforming growth factor (TGF)-β1, interleukin(IL)-6 and matrix metalloproteinase (MMP)-1 in
wounds were determined by enzyme-linked immunosorbent assay (ELISA). In vitro study evaluated the influence of carboxymethyl-chitosan on cytokines secretion of fibroblasts and macrophages. In vivo results showed that carboxymethyl-chitosan effectively accelerated the wound healing process in burned rats (P<0.05). Levels of TGF-β1, IL-6 and MMP-1 in carboxymethyl-chitosan groups were significantly elevated, compared with control
group (P<0.05). In vitro results indicated that carboxymethyl-chitosan significantly promoted the proliferation of fibroblasts and stimulated its
IL-8 and IL-10 secretion at different incubation time, but it did not affect collagen secretion of fibroblasts. Carboxymethyl-chitosan
enhanced phagocytosis ability of macrophages, and increased its tumor necrosis factor (TNF)-α secretion. In conclusion, carboxymethyl-chitosan
promoted wound healing by activating macrophages, accelerating fibroblasts growth and exerting considerable effects on the
secretion of a series of cytokines. |
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| Keywords: | carboxymethyl-chitosan wound healing fibroblasts macrophages cytokines |
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