| 川纹笛鲷染色体核型、银染和C-带 |
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| 引用本文： | 郭明兰,游欣欣,苏永全,丁少雄,王军.川纹笛鲷染色体核型、银染和C-带[J].热带海洋学报,2011,30(1). |
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| 作者姓名： | 郭明兰 游欣欣 苏永全 丁少雄 王军 |
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| 作者单位： | 1. 中国科学院南海海洋研究所海洋生物资源可持续利用重点实验室,广东,广州,510301
2. 厦门大学海洋与环境学院,福建厦门,361005 |
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| 基金项目： | 国家自然科学基金项目，国家"863"计划项目 |
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| 摘 要： | 实验采用植物血凝素、秋水仙碱腹腔注射,肾细胞直接制片法分析了川纹笛鲷Lutjanus sebae染色体核型、Ag-NORs和C-带.结果表明:1)川纹笛鲷二倍体染色体数2n=48,核型公式为:2n=48t,NF=48;在第1对染色体靠近着丝粒部位有明显的次缢痕.2)染色体经快速银染后,Ag-NORs的数目在不同细胞中表现出多态性,数目1-4个,2个Ag-NORs的频率最高(占79%).在分裂相中,第1对t染色体近着丝粒的次缢痕区均出现2个银染位点(Ag-NORs阳性),且未见Ag-NORs的联合现象.3)大多数染色体的着丝粒区显示出1个深浅不同的C-带,在第1对染色体的随体区域分布有大量的结构异染色质,表现C-带强阳性.讨论了鱼类核型演化规律和Ag-NORs,C-带的发生机制,以及川纹笛鲷的进化地位.
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| 关 键 词： | 川纹笛鲷Lutjanus 核型 C-带 |
Studies on chromosome karyotype, Ag-NORs and C-banding patterns of Lutjanus sebae |
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| GUO Ming-lan,YOU Xin-xin,SU Yong-quan,DING Shao-xiong,WANG Jun.Studies on chromosome karyotype, Ag-NORs and C-banding patterns of Lutjanus sebae[J].Journal of Tropical Oceanography,2011,30(1). |
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| Authors: | GUO Ming-lan YOU Xin-xin SU Yong-quan DING Shao-xiong WANG Jun |
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| Institution: | GUO Ming-lan1,YOU Xin-xin2,SU Yong-quan2,DING Shao-xiong2,WANG Jun2(1.Key Laboratory of Marine Bio-resources Sustainable Utilization,South China Sea Institute of Oceanology,CAS,Guangzhou 510301,China,2.Department of Oceanography and Institute of Subtropical Oceanography,Xiamen University,Xiamen 361005,China) |
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| Abstract: | Studies on karyotype,Ag-NORs,and C-banding of Lutjanus sebae were performed.The chromosomes were re-ceived from the head kidney by using a method of injecting with PHA and colchicines,air drying,and Giemsa staining.The results were as follows.1) The karyotypic formula of L.sebae was 2n=48t,NF=48.Meanwhile,a pair of chromosomes,with secondary constriction near the centromere,was found on chromosome 1.2) The Ag-NORs polymorphisms were individually specific in this fish.The silver staining pots were 1-4,and th... |
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| Keywords: | sebae Ag-NORs |
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