| 球等鞭金藻细胞生长抑制因子的初步研究 |
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| 引用本文： | 王长海,孙颖颖.球等鞭金藻细胞生长抑制因子的初步研究[J].海洋通报,2006,25(3):29-33. |
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| 作者姓名： | 王长海 孙颖颖 |
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| 作者单位： | 1. 烟台大学海洋学院,山东,烟台,264005;大连理工大学环境与生命科学学院,辽宁,大连,116024
2. 大连理工大学环境与生命科学学院,辽宁,大连,116024 |
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| 摘 要： | 以球等鞭金藻老化培养液的乙酸乙酯粗提物处理微藻细胞,通过对细胞密度、叶绿素a含量、胞内多糖含量及蛋白质含量等生理指标的测定,证实了乙酸乙酯粗提物对球等鞭金藻细胞生长有明显的抑制作用。进一步比较乙酸乙酯粗提物对球等鞭金藻细胞密度、叶绿素a含量及胞内多糖含量96h的半抑制剂量,得出细胞密度、胞内多糖及叶绿素a可以作为反映乙酸乙酯粗提物对球等鞭金藻抑制作用的生物学指标。
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| 关 键 词： | 微藻 球等鞭金藻 乙酸乙酯粗提物 生长抑制因子 生长 |
| 文章编号： | 1001-6392（2006）03-0029-05 |
| 收稿时间： | 2005-04-04 |
| 修稿时间： | 2005-04-042005-06-07 |
Researches on the Growth Inhibitor in Culture of Isochrysis galbana |
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| WANG Changhai,SUN Yingying.Researches on the Growth Inhibitor in Culture of Isochrysis galbana[J].Marine Science Bulletin,2006,25(3):29-33. |
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| Authors: | WANG Changhai SUN Yingying |
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| Institution: | 1. Yantai University, Yantai 264005, Shandong, China; 2. Dalian University of Technology, Dalian 116024, Liaoning, China |
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| Abstract: | The crude inhibitor, ethyl acetate extracts, was obtained from the old culture liquid of Isochrysis galbana. The cultures were treated by ethyl acetate extracts in different concentration, and then the effects of this extract on the cell density, chlorophyll a, polysacchrides and proteins were investigated. The results showed that the microalgal cells were sensitive to this crude inhibitor, and the cell density, polysacchrides and chlorophyll a could be used as biological marks for researching the inhibitory affect on the growth of Isochrysis galbana. |
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| Keywords: | mircoalgae Isochrysis galbana ethyl acetate extract inhibitor growth |
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