ISSR analysis of Caragana microphylla (Leguminosae) in
different temperature gradients |
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Authors: | WenDa Huang XueYong Zhao YuLin Li YuQiang Li YaYong Luo Jing Feng and Na Su |
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Institution: | Naiman Desertification Research Station, Cold and Arid Regions Environmental and Engineering Research Institute,
Chinese Academy of Sciences, Lanzhou, Gansu 730000, China;Naiman Desertification Research Station, Cold and Arid Regions Environmental and Engineering Research Institute,
Chinese Academy of Sciences, Lanzhou, Gansu 730000, China;Naiman Desertification Research Station, Cold and Arid Regions Environmental and Engineering Research Institute,
Chinese Academy of Sciences, Lanzhou, Gansu 730000, China;Naiman Desertification Research Station, Cold and Arid Regions Environmental and Engineering Research Institute,
Chinese Academy of Sciences, Lanzhou, Gansu 730000, China;Naiman Desertification Research Station, Cold and Arid Regions Environmental and Engineering Research Institute,
Chinese Academy of Sciences, Lanzhou, Gansu 730000, China;Naiman Desertification Research Station, Cold and Arid Regions Environmental and Engineering Research Institute,
Chinese Academy of Sciences, Lanzhou, Gansu 730000, China;Naiman Desertification Research Station, Cold and Arid Regions Environmental and Engineering Research Institute,
Chinese Academy of Sciences, Lanzhou, Gansu 730000, China |
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Abstract: | Caragana microphylla is the most dominant and constructive shrub species in the Horqin Sandy Land of northeastern
China. We evaluated the level of genetic variation within and among C. microphylla populations sampled from three
different temperature gradients in the Horqin Sandy Land by using inter-simple sequence repeat polymorphism (ISSR)
molecular markers. The results show that eight ISSR primers generated 127 bands, of which 123 (96.85%) were
polymorphic. At the species level, genetic diversity was relatively high (P = 96.85%, h = 0.3143, I = 0.4790). The
highest genetic diversity was observed in the Subp6 population from low temperature regions, whereas the lowest diversity
was found in the Subp2 population from high temperature regions. Six populations of C. microphylla clustered
into two clades. These results have important implications for restoring and managing the degraded ecosystem in arid
and semi-arid areas. |
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Keywords: | Caragana microphylla temperature gradients ISSR Horqin Sandy Land |
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