Analysis of SSRs derived from an EST library of half-smooth tongue sole <Emphasis Type="Italic">Cynoglossus semilaevis</Emphasis> |
| |
| Authors: | Yunguo Liu Baolong Bao Songlin Chen Xiuqin Sun |
| |
| Institution: | (1) Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao, Shandong, 266071, China;(2) Department of Soil and Crop Sciences and Institute for Plant Genomics and Biotechnology, Texas A&;M University, College Station, TX 77843, USA; |
| |
| Abstract: | A complementary DNA (cDNA) library was constructed from half-smooth tongue sole spleen. A long-read expressed sequence tag
(EST) database was generated, containing 3100 cDNA clones, of which 220 clones were fully sequenced. A total of 1060 non-redundant
simple sequence repeats (SSRs) were obtained from the cDNA library. An average of 5 kb sequence generates 1 SSR in the half-smooth
tongue sole spleen cDNA library. The proportion of the SSR unit size was different in the cDNA library. The monomeric repeats
(51.4%) are the most abundant class of SSR in the dataset. The dimeric, trimeric, tetrameric and hexameric repeats are represented
in decreasing proportions of 27.2%, 16.0%, 2.8% and 1.9%, respectively. The frequency of pentameric repeats was observed the
least (only 0.7%). Most of the monomeric and dimeric repeats are distributed in 3′ and 5′ un-translation region. If translation
regions are considered merely, trimeric repeats are the highest, accounting for 57% of the total microsatellites. |
| |
| Keywords: | half-smooth tongue sole Cynoglossus semilaevis EST microsatellite |
| 本文献已被 SpringerLink 等数据库收录！ |
|
