| 甲壳胺寡糖的液相色谱及薄层层析分析 |
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| 引用本文： | 房子,刘万顺,位晓娟,韩宝芹.甲壳胺寡糖的液相色谱及薄层层析分析[J].中国海洋大学学报(自然科学版),2005,35(1):113-115,120. |
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| 作者姓名： | 房子 刘万顺 位晓娟 韩宝芹 |
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| 作者单位： | 中国海洋大学海洋生命学院,山东,青岛,266003 |
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| 基金项目： | “十五”国家科技攻关项目 (2 0 0 1BA70 8B0 4 0 7)资助 |
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| 摘 要： | 为了解低分子甲壳胺多糖的抗肿瘤辅助疗效，作者利用高效液相色谱法(HPLC)结合薄层色谱法(TLC)，探讨了SUGAR-KS-802寡糖分析柱分析甲壳胺寡糖的条件，并尝试了几种薄层层析展开剂对甲壳胺寡糖组分的展层条件。结果表明，在HPLC流动相为0．001mol／L NaOH溶液，流速0．2mL／min时，可将混合的标准甲壳胺寡糖成分很好地分离；采用乙酸乙脂：甲醇：水：氨水=5：9：1：1．5混合液作为硅胶薄板展开剂时，可将不同聚合度的甲壳胺寡糖很好地分开，且层析重现性高，糖残基数目和Rf值具有很好的线性关系。2种分析方法结合可以作为1种可靠的甲壳胺寡糖分析方法。
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| 关 键 词： | HPLC TLC 甲壳胺寡糖 |
| 文章编号： | 1672-5174(2005)01-113-03 |
The Separation of Chito-Oligosaccharides by HPLC with TLC |
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| FANG Zi,LIU Wan-shun,WEI Xiao-juan,HAN Bao-qin.The Separation of Chito-Oligosaccharides by HPLC with TLC[J].Periodical of Ocean University of China,2005,35(1):113-115,120. |
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| Authors: | FANG Zi LIU Wan-shun WEI Xiao-juan HAN Bao-qin |
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| Abstract: | The standard chitosan-oligosaccharides were separated by using a novel SUGAR-KS-802 column and TLC methods. The optimal solvent system and HPLC conditions were found. Chitosan-oligosaccharides can be easily separated by using the SUGAR-KS-802 column under the condition of 0.001mol/L NaOH at 0.2mL/min, and chitosan-oligosaccharides ranging from dp1 to dp6 can be clearly separated with TLC using ethyl acetate/methano/water/ammonia water in 5/9/1/1.5 as developing system.The combination of HPLC and TLC analysis is a reliable method for the analysis and separation of chitosan-oligosaccharides. |
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| Keywords: | HPLC TLC(thin-layer chromatograph) chito-oligosaccharide |
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