| Gene cloning and sequence analysis of the cold-adapted chaperones DnaK and DnaJ from deep-sea psychrotrophic bacterium Pseudoalteromonas sp.SM9913 |
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| 作者姓名： | ZHAO Dianli CHEN Xiulan HE Hailun SHI Mei ZHANG Yuzhong |
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| 作者单位： | ZHAO Dianli1，CHEN Xiulan1，HE Hailun1，SHI Mei1，ZHANG Yuzhong1 1.State Key Laboratory of Microbial Technology，Marine Biotechnology Research Center，Shandong University，Jinan 250100，China |
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| 基金项目： | 国家高技术研究发展计划(863计划)
,
the China Ocean Mineral Resources R & D Association
,
国家自然科学基金
,
山东省自然科学基金
,
山东省杰出中青年科学家奖励基金
,
教育部高校新世纪杰出人才项目 |
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| 摘 要： | Pseudoalteromonas sp.SM9913 is a phychrotrophic bacterium isolated from the deep-sea sediment.The genes encoding chaperones DnaJ and DnaK of P.sp.SM9913 were cloned by normal PCR and TAIL-PCR(GenBank accession Nos DQ640312,DQ504163).The chaperones DnaJ and DnaK from the strain SM9913 contain such conserved domains as those of many other bacteria,and show some cold-adapted characteristics in their structures when compared with those from psychro-,meso-and themophilic bacteria.It is indicated that chaperones DnaJ and DnaK of P.sp.SM9913 may be adapted to low temperature in deep-sea and function well in assisting folding,assembling and translocation of proteins at low temperature.This research lays a foundation for the further study on the cold-adapted mechanism of chaperones DnaJ and DnaK of cold-adapted microorganisms.
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| 关 键 词： | 深海细菌 基因克隆 序列分析 基因编码 |
| 收稿时间： | 2006-09-12 |
| 修稿时间： | 2007-03-15 |
Gene cloning and sequence analysis of the cold-adapted chaperones DnaK and DnaJ from deep-sea psychrotrophic bacterium Pseudoalteromonas sp.SM9913 |
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| ZHAO Dianli,CHEN Xiulan,HE Hailun,SHI Mei,ZHANG Yuzhong.Gene cloning and sequence analysis of the cold-adapted chaperones DnaK and DnaJ from deep-sea psychrotrophic bacterium Pseudoalteromonas sp.SM9913[J].Acta Oceanologica Sinica,2007,26(6):91-100. |
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| Authors: | ZHAO Dianli CHEN Xiulan HE Hailun SHI Mei and ZHANG Yuzhong |
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| Institution: | State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, China |
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| Abstract: | Pseudoalteromonas sp. SM9913 is a phychrotrophic bacterium isolated from the deep-sea sediment. The genes encoding chaperones DnaJ and DnaK of P. sp. SM9913 were cloned by normal PCR and TAIL-PCR (GenBank accession Nos DQ640312, DQ504163). The chaperones DnaJ and DnaK from the strain SM9913 contain such conserved domains as those of many other bacteria, and show some cold-adapted characteristics in their structures when compared with those from psychro-, meso-and themophilic bacteria. It is indicated that chaperones DnaJ and DnaK of P. sp. SM9913 may be adapted to low temperature in deep-sea and function well in assisting folding, assembling and translocation of proteins at low temperature. This research lays a foundation for the further study on the cold-adapted mechanism of chaperones DnaJ and DnaK of cold-adapted microorganisms. |
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| Keywords: | cold-adapted chaperone DnaJ DnaK deep-sea Pseudoalteromonas sp SM9913 gene cloning sequence analysis |
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