| Abstract: | Tissue culture of farmed and wild shrimp (Penaes e chinensis) was studied. The vacuolization of migrated cells from cultured ti ssue and many granules in them were observed. These attached cells of farmed shr imp gradually came off after 7 days culture. The cells of wild shrimp could be a live for 30 days or so. We examined the lymphoid tissues of shrimp and cultured tissues in vitro with transmission electronic microscope (TEM). Besides a ki nd of spherical virus and two kinds of virus inclusion, a large number of Ricket tsia-like organisms were found in the lymphoid tissues of farmed shrimp. While in the lymphoid tissues of wild shrimp, a few spherical viruses, a kind of globa l virus inclusion and Rickettsia-like organisms were also found in a few cells. But some cells of wild shrimp infected by the Rickettsia-like organisms showed pycnosis. Pathological changes, including vacuolization, nuclear membrane hyper trophy, nuclear chromatin pycnosis, Golgi body swelling, cristae of mitochondria disappearing were observed in cells infected by virus. Those cells infected by Rickettsia-like organisms showed organelle disappearing and chromosome pycnosis . |
