Serum immunoglobulin of the mandarin fish, Siniperca chuatsi with development of polyclonal antibody |
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| Authors: | Zhang Yong-an and Nie Pin |
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| Institution: | (1) State Key Laboratory of Freshwater Ecology and Biotechnology, and Laboratory of Fish Diseases, Institute of Hydrobiology, Chinese Academy of Sciences, 430072 Wuhan, China |
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| Abstract: | Serum immunoglobulin from the mandarin fish, or the so-called Chinese perch,Siniperca chuatsi (Basilewsky), was successfully purified using affinity chromatography. Heavy and light chains were detected on electrophoresis
gel, with molecular weights being estimated at 72 and 29 kDa, respectively. The tetrameric IgM ofS. chuatsi was calculated to be 808 kDa. The rabbit polyclonal antisera against the purifed immunoglobulin were developed and tested
by Western blot analysis. The antisera reacted strongly with the heavy chains ofS. chuatsi immunoglobulin. Humoral immune responses of the mandarin fish can then be examined using the developed polyclonal antibody.
Project No. 30025035 supported by NSFC, State Key Laboratory of Freshwater Ecology and Biotechnology (2000FB02), and the Chinese
Academy of Sciences (KZ9511-B1-111). |
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| Keywords: | immunoglobulin polyclonal antibody mandarin fish Chinese perch Siniperca chuatsi |
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