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| 螺旋藻多糖对肿瘤细胞生长及Hela细胞早期凋亡作用的实验研究 | |
| 引用本文： | 于 红,吕 锐,张学成.螺旋藻多糖对肿瘤细胞生长及Hela细胞早期凋亡作用的实验研究[J].海洋科学,2008,32(1):38-40. |
| 作者姓名： | 于 红 吕 锐 张学成 |
| 作者单位： | 1. 中国科学院,东北地理与农业生态研究所,吉林,长春,130012 2. 吉林省水产技术推广总站,吉林,长春,130012 3. 辽宁省营口市水产科学研究所,辽宁,营口,115000 |
| 基金项目： | 吉林省科技发展计划资助项目(20020227-2) |
| 摘 要： | 采用MTT法(四甲基偶氮唑盐比色法)研究了钝顶螺旋藻多糖(PSP)对Hela细胞及HepG2细胞生长的抑制作用。结果表明,随PSP浓度及培养时间的增加,肿瘤细胞存活率逐渐降低,抑制率逐渐增加,以PSP40 mg/L作用72 h最为显著。应用Annexin V/PI双染色流式细胞仪检测了早期Hela细胞凋亡,未经PSP处理的正常Hela细胞凋亡细胞极少,经PSP处理的细胞,凋亡细胞的百分比明显高于正常对照组,其作用随着剂量的增加和时间的延长而增强,具有量效关系和时效关系。结果说明PSP的抗肿瘤机制,除了诱导肿瘤细胞凋亡,还存在细胞毒性等其他机制,是多种机制共同作用的结果。 |
| 关 键 词： | 钝顶螺旋藻多糖 抗肿瘤作用 细胞凋亡 AnnexinV/PI |
| 文章编号： | 1000-3096（2008）01-0038-03 |
| 收稿时间： | 2005-12-20 |
| 修稿时间： | 2006-02-10 |
Experimental studies on antiproliferation activities and early apoptosis inducement of polysaccharides from Spirulina platensis |
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| YANG Fu-yi,LI Xiu-jun,YANG Xin-qiao,SUN Li-min.Experimental studies on antiproliferation activities and early apoptosis inducement of polysaccharides from Spirulina platensis[J].Marine Sciences,2008,32(1):38-40. | |
| Authors: | YANG Fu-yi LI Xiu-jun YANG Xin-qiao SUN Li-min |
| Abstract: | In vitro experiments the antiproliferation activities of polysaccharides from Spirulina platensis(PSP) were evaluated on Hela cells and HepG2 cells by MTT assay,the results showed that PSP(2.5~40 mg/L,48~72 h) exhibited dose and time inhibition effects on the cell growth.The early apoptosis of Hela cells was detected by flow cytometry analysis with a combination of Annexin V-FITC and PI double staining,the apoptosis rates induced by PSP(5,10,20,40 mg/L) were 30.3%,32.6%,34.8% and 38.8% respectively,which were higher than those of the normal control group(P<0.05).Meanwhile,the apoptosis rate treated by PSP(40 mg/L) also increased in time-dependent manner.These results suggested that one of the anti-tumor mechanisms of PSP may be related to inhibition on cell proliferation and induction of notable apoptosis. |
| Keywords: | polysaccharides from Spirulina platensis(PSP) antitumor effect apoptosis annexin V/PI |
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