| 三疣梭子蟹(Portunus trituberculatus)Pthyastatin抗菌肽基因的克隆与表达分析 |
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| 引用本文： | 申 望,叶 茂,石 戈,王日昕.三疣梭子蟹(Portunus trituberculatus)Pthyastatin抗菌肽基因的克隆与表达分析[J].海洋与湖沼,2010,41(2):246-253. |
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| 作者姓名： | 申 望 叶 茂 石 戈 王日昕 |
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| 作者单位： | 海洋生物资源及分子工程实验室,浙江海洋学院海洋科学学院,舟山,316004 |
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| 基金项目： | 国家科技支撑计划项目,2007BAD43B08号;;浙江省重大科技专项重大项目,2007C02001号;;浙江省面上项目,2009C32019号;;浙江省教育厅项目,20061141号 |
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| 摘 要： | 采用构建三疣梭子蟹血细胞全长cDNA文库的方法克隆了一个新型抗菌肽基因Pthyastatin,并用荧光定量RT-PCR方法,进行Pthyastatin的表达研究。结果表明,Pthyastatin前体由16个氨基酸残基的信号肽和成熟肽两部分组成,其中Pthyastatin成熟肽有两个结构域:N端的富含Pro/Arg结构域和C端的包含6个保守Cys残基与对虾抗菌肽penaeidins同源的结构域,表明Pthyastatin属于对虾抗菌肽penaeidins家族;荧光定量RT-PCR分析结果表明Pthyastatin在血细胞中高水平组成型表达;致病菌副溶血弧菌诱导后Pthyastatin在血细胞中表达先下调、后上调,在诱导后24h表达量又基本恢复到诱导前水平,支持Pthyastatin在血细胞中高水平组成型表达的观点,但致病菌诱导后表达变化机制不明。
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| 关 键 词： | 三疣梭子蟹 血细胞 抗菌肽 Pthyastatin mRNA表达 |
| 收稿时间： | 2009/6/19 0:00:00 |
| 修稿时间： | 2009/8/25 0:00:00 |
cDNA CLONING, CHARACTERIZATION AND mRNA EXPRESSION OF A HYASTATIN-LIKE GENE FROM SWIMMING CRAB PORTUNUS TRITUBERCULATUS |
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| SHEN Wang,YE Mao,SHI Ge and WANG Ri-Xin.cDNA CLONING, CHARACTERIZATION AND mRNA EXPRESSION OF A HYASTATIN-LIKE GENE FROM SWIMMING CRAB PORTUNUS TRITUBERCULATUS[J].Oceanologia Et Limnologia Sinica,2010,41(2):246-253. |
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| Authors: | SHEN Wang YE Mao SHI Ge and WANG Ri-Xin |
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| Institution: | Laboratory of Marine Biology Resources and Molecular Engineering;Ocean Science College;Zhejiang Ocean University;Zhoushan;316004 |
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| Abstract: | Pathogenic challenges in decapod crustaceans are combated by innate immune responses, including the production and secretion of soluble antibacterial peptides into hemolymph. Hyastatin antimicrobial homologues, containing a domain of six Cys residues at the C-terminus, had been identified from the haemocyte library of the swimming crab, Portunus trituberculatus and designated Pthyastatin. Sequence analysis of these cDNAs indicated the presence of five isoforms of Pthyastatin. The full-length cDNA of Pthyast... |
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| Keywords: | Portunus trituberculatus Hemocyte Antimicrobial peptide Pthyastatin mRNA expression |
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