| 极地产适冷脂肪酶菌株的酶学性质研究 |
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| 引用本文： | 陈兴麟,翁金樑,曾润颖,刘光明.极地产适冷脂肪酶菌株的酶学性质研究[J].台湾海峡,2010,29(1):12-19. |
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| 作者姓名： | 陈兴麟 翁金樑 曾润颖 刘光明 |
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| 作者单位： | 1. 国家海洋局第三海洋研究所、国家海洋局生物与遗传资源重点实验室,福建,厦门,361005
2. 集美大学生物工程学院,福建,厦门,361021 |
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| 基金项目： | 国家863计划资助项目 |
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| 摘 要： | 从南北极环境样品中分离到7株产脂肪酶的细菌，经16SrDNA序列分析表明，这些细菌分别属于假交替单胞菌属（Pseudoalteromonas）和嗜冷杆菌属（Psychrobacter）．采用p-NPP法对这7株细菌所产的脂肪酶进行酶学性质研究，结果显示这些酶的最适作用温度在30～40cC、最适作用pH值在7—8之间，在低温下能保持较高的剩余活力，对热敏感，属于适冷脂肪酶．其中假交替单胞菌（Psychrobacter sp．7342）所产脂肪酶具有低温下酶的剩余活力高、有效作用温度和pH范围广、热稳定性较好及对多种金属离子抗性强等特点．该菌株能利用多种单一氮源和碳源产酶，最适产酶温度为25℃．在此基础上进行正交实验分析得到了该菌株的最佳发酵条件为：蛋白胨和淀粉含量各为1．33％，酵母膏含量为0．3％，温度为25℃．
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| 关 键 词： | 海洋微生物学 适冷脂肪酶 酶学性质 发酵条件 极地 |
Research of enzyme characters of cold-adapted lipase-produced bacteria from the polar |
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| CHEN Xing-lin,WENG Jin-liang,ZENG Run-ying,LIU Guang-ming.Research of enzyme characters of cold-adapted lipase-produced bacteria from the polar[J].Journal of Oceanography In Taiwan Strait,2010,29(1):12-19. |
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| Authors: | CHEN Xing-lin WENG Jin-liang ZENG Run-ying LIU Guang-ming |
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| Institution: | CHEN Xing-lin1,WENG Jin-liang2,ZENG Run-ying1,LIU Guang-ming2(1.Key Laboratory for Marine Biogenetic Resources,SOA,Xiamen 361005,China,2.College of Biological Engineering,Jimei University,Xiamen 361021,China) |
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| Abstract: | Seven strains of lipase-produced bacteria were isolated and cultured from soil samples of Antarctic and arctic.The results of 16S rDNA sequence analysis showed that 7 bacteria belong to Pseudoalteromonas sp.and Psychrobacter sp.Their enzyme products of bacteria were analysed by p-NPP method.The results showed that optimal temperature of enzyme was 30～40℃,the optimal pH was 7～8.The enzyme maintained a high surplus vitality at low temperature and was heat-sensitive.So the enzyme belonged to low-temperature lipases.The lipase produced by Psychrobacter sp.7342 maintained high enzyme activity at low temperature,had an effective role in a wide range of temperature and pH.It also had better thermal stability and was resisted to a variety of metal ions.The strain can use a variety of single carbon and nitrogen produce enzyme,the optimum temperature for enzyme production is 25℃.Based on the orthogonal experiment,the best fermentation condition of the strain was preliminarily found to be:Tryptone 1.33%,starch 1.33%,yeast 0.3%,at 25℃. |
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| Keywords: | marine microbiology cold-adapted lipase characterization of enzyme fermentation condition polar region |
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