| 褐点石斑鱼不同组织4种同工酶的研究 |
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| 引用本文： | 尹绍武,廖经球,黄海,雷从改,陈国华,张本.褐点石斑鱼不同组织4种同工酶的研究[J].海洋通报,2007,26(1):41-44. |
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| 作者姓名： | 尹绍武 廖经球 黄海 雷从改 陈国华 张本 |
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| 作者单位： | 热带生物资源教育部重点实验室,海南,海口,570228;海南大学海洋学院,海南,海口,570228 |
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| 基金项目： | 海南省教育厅高校科学研究资助项目(HjKj200513)，海南大学科研资助项目(Kyjj0439) |
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| 摘 要： | 运用聚丙烯酰胺垂直梯度凝胶电泳法对褐点石斑鱼7种组织(眼睛、肌肉、心脏、肝脏、肾脏、尾鳍、脾脏)的4种同工酶(EST、SOD、LDH、MDH)进行了初步研究。结果表明:褐点石斑鱼的4种同工酶系统具有不同程度的组织特异性。EST由4个基因位点编码,具有多态性现象;肌肉SOD与其它组织SOD有明显差异;眼睛LDH酶谱酶带最多;MDH具有线粒体型(m-MDH)和上清液型(s-MDH)两种类型。
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| 关 键 词： | 褐点石斑鱼 同工酶 电泳 组织 |
| 文章编号： | 1001-6932（2007）01-0041-0044 |
| 修稿时间： | 2005-10-172005-12-29 |
Studies on Four Kinds of Isozyme in Different Tissues of Epinephelus fuscoguttatus |
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| YIN Shaowu,LIAO Jingqiu,HUANG Hai,LEI Conggai,CHEN Guohua,ZHANG Ben.Studies on Four Kinds of Isozyme in Different Tissues of Epinephelus fuscoguttatus[J].Marine Science Bulletin,2007,26(1):41-44. |
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| Authors: | YIN Shaowu LIAO Jingqiu HUANG Hai LEI Conggai CHEN Guohua ZHANG Ben |
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| Institution: | 1. Key Laboratory of Tropical Biology Resources, Ministry of Education, Haikou 570228, Hainan, China; 2. College of Ocean, Hainan University, Haikou 570228, Hainan, China |
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| Abstract: | Four isozymes (EST, SOD, LDH and MDH) from seven tissues (eye, muscle, heart, liver, kidney, caudal fin and spleen) of E. fuscoguttatus were preliminarily studied by using vertical polyacrylamide gel gradient electrophoresis. The results showed that the four isozymes presented the tissue specificityto varying degrees. The number of EST isoenzyme bands was 15 EST, which was encoded by 4 loci, and had strong polymorphism. EST-5 ,EST-9, EST-10 and EST-15 existed in all 7 tissues. The number of SOD isoenzyme bands was 19 and the band model was different from many fish. SOD in muscle was very special. LDH isoenzyme bands were few. The most specific LDH was in eye that had 7 isoenzyme bands and was encoded by 2 loci. Two types of MDH (m-MDH and s-MDH) were found from E. fuscoguttatus. The isoenzyme bands of m-MDH-1 - m-MDH-3 also existed in all 7 tissues. |
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| Keywords: | Epinephelus fuscoguttatus isozyme gel electrophoresis tissues |
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