| 中国对虾淋巴组织的长期原代培养和化学诱变初探 |
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| 引用本文： | 郎刚华,王勇,汪岷,陈西广.中国对虾淋巴组织的长期原代培养和化学诱变初探[J].中国海洋大学学报(自然科学版),2001,31(3):411-414. |
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| 作者姓名： | 郎刚华 王勇 汪岷 陈西广 |
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| 作者单位： | 1. 青岛海洋大学海洋生命学院,
2. 中国科学院海洋研究所, |
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| 基金项目： | 国家海洋 86 3项目!(819- 0 4- 0 4)资助 |
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| 摘 要： | 以中国对虾为实验材料 ,研究影响对虾淋巴组织原代培养的一些因素。研究中发现 ,用75%的乙醇浸泡对虾就可以达到淋巴组织培养无菌的要求 ,当组织块的接种密度为 2 4 0～ 2 60块( 2 5cm2 ) -1时最适。当原代细胞培养 30 d左右 ,细胞会因接触抑制而停止生长 ,当接触抑制消除后 ,细胞仍可继续生长。另外 ,还进行了以 N-甲基 - N′-亚硝基胍 ( MNNG)对原代细胞进行诱变的尝试发现诱变后有的细胞呈现出转化细胞的形态 ,并形成了转化灶
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| 关 键 词： | 中国对虾 淋巴组织 组织培养 原代培养 化学诱变 N-甲基-N′-亚硝基胍(MNNG) |
| 文章编号： | 1001-1862(2001)03-411-04 |
| 修稿时间： | 2000年6月30日 |
The Long-Term Primary Culture of Penaeus Chinensis Lymphoid Tissue and a Preliminary Study of its Chemical Transformation |
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| Lang Ganghua,Wang Yong,Wang Min,Chen Xiguang.The Long-Term Primary Culture of Penaeus Chinensis Lymphoid Tissue and a Preliminary Study of its Chemical Transformation[J].Periodical of Ocean University of China,2001,31(3):411-414. |
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| Authors: | Lang Ganghua Wang Yong Wang Min Chen Xiguang |
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| Institution: | Lang Ganghua Wang Yong 1\ Wang Min\ Chen Xiguang |
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| Abstract: | Some factors affecting the primary culture of Penaeus chinensis lymphatic cells were studied. It was found that simply rinsing the shrimp in 75% ethanol could obtain satisfactory result in sterilization, the optimal inoculating density was 240~260 blocks/25cm 2. After cultured for about 30 days, the cells stopped growing due to contact inhibition. However, after the inhibition was broken by scratching away some cells, the cells continued to grow. Moreover, after we had tried to mutagenize the cells with N methyl N` nitrosoguanidine (MNNG), some transformed cells had some morphological changes and grew in multilayer. In the meantime, many foci turned up. |
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